Construction of Salmonella strains with both antigen O4 (of group B) and antigen O9 (of group D).
Construction of Salmonella strains with both antigen O4 (of group B) and antigen O9 (of group D).
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构建具有抗原 O4（B 组）和抗原 O9（D 组）的沙门氏菌菌株。
DOI：
10.1128/jb.174.6.1911-1915.1992
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发表时间：
1992
影响因子：
3.2
通讯作者：
Stocker,BA
中科院分区：
文献类型：
--
作者：
Johnson,BN;Weintraub,A;Lindberg,AA;Stocker,BA
沙门氏菌活疫苗引起O 4和O 9特异性免疫反应将是有用的，但没有报道的沙门氏菌血清型具有这两种O抗原。构建的鼠伤寒沙门氏菌菌株在染色体中具有D型rfb（O-抗原特异性）基因簇，在F '-rfb+因子中具有B型基因簇，以及具有反向组合的菌株与抗-O 4（和抗-O 5）和抗-O 9血清均发生强烈反应，如果它们携带recA，则可以通过选择性保留F'因子的生长条件维持在这种状态。一株（P22-溶原性）S.具有包括hisD和B.rfb+的染色体片段的串联重复的鼠伤寒沙门氏菌菌株被D.rfb+基因簇替换（通过转导）;所得菌株为O 1 + O 4 + O 5 + O 9+，并且在制备recA后本身稳定。一种稳定的O_4 ~+ O_9 ~+衍生物。通过首先将合适的串联重复菌株的连接点与相邻的B.rfb+基因簇一起转导入其中，然后将srl：：Tn 10 recA转导入其中，制备了重组（O-组D）菌株。
A Salmonella live vaccine causing both O4- and O9-specific immune responses would be of use, but no reported Salmonella serotype has both of these O antigens. Constructed Salmonella typhimurium strains with an rfb (O-antigen-specifying) gene cluster of type D in the chromosome and one of type B in an F'-rfb+ factor, and those with the reverse combination reacted strongly with both anti-O4 (and anti-O5) and anti-O9 sera and, if they carried recA, could be maintained in this state by growth conditions selective for retention of the F' factor. One of the two B.rfb+ gene clusters of a (P22-lysogenic) S. typhimurium strain with a tandem duplication of a chromosomal segment including hisD and B.rfb+ was replaced (by transduction) by a D.rfb+ gene cluster; the resulting strain was O1+ O4+ O5+ O9+ and stable as such after being made recA. A stable O4+ O9+ derivative of a virulent S. enteritidis (O-group D) strain was made by transducing into it first the join point of an appropriate tandem duplication strain, together with the adjacent B.rfb+ gene cluster, and then srl::Tn10 recA.