Design and fabrication of tubular scaffolds via direct writing in a melt electrospinning mode.
Design and fabrication of tubular scaffolds via direct writing in a melt electrospinning mode.
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DOI：
10.1007/s13758-011-0013-7
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发表时间：
2012-12
期刊：
影响因子：
2.1
通讯作者：
Hutmacher DW
中科院分区：
文献类型：
--
作者：
Brown TD;Slotosch A;Thibaudeau L;Taubenberger A;Loessner D;Vaquette C;Dalton PD;Hutmacher DW
由溶液电纺纤维制成的柔性管状结构在组织工程应用中得到了越来越多的应用。然而，由于溶液电纺射流的混沌性质，很难控制纤维的沉积。通过使用非导电聚合物熔体而不是聚合物溶液，纤维的路径和收集变得可预测。在这项工作中，我们演示了聚己内酯的熔融电纺在旋转的圆柱体上的直接写入模式。这使得可以使用直径20μm的纤维设计和制造具有可控微图案和机械性能的管。一个关键的设计参数是纤维缠绕角度，它允许控制支架的孔形态(例如，大小、形状、数量和孔隙率)。此外，将建立的有限元模型作为预测设计工具与熔体电纺管材的力学测试结果进行了验证，结果表明，较小的缠绕角可以改善单轴拉伸和压缩的力学响应。此外，我们发现，熔融电纺管在体外支持三种不同类型的细胞生长，因此是一种有前景的组织工程应用支架。本文的在线版本(doi：10.1007/s13758-0110013-7)包含补充材料，可供授权用户使用。
Flexible tubular structures fabricated from solution electrospun fibers are finding increasing use in tissue engineering applications. However it is difficult to control the deposition of fibers due to the chaotic nature of the solution electrospinning jet. By using non-conductive polymer melts instead of polymer solutions the path and collection of the fiber becomes predictable. In this work we demonstrate the melt electrospinning of polycaprolactone in a direct writing mode onto a rotating cylinder. This allows the design and fabrication of tubes using 20 μm diameter fibers with controllable micropatterns and mechanical properties. A key design parameter is the fiber winding angle, where it allows control over scaffold pore morphology (e.g. size, shape, number and porosity). Furthermore, the establishment of a finite element model as a predictive design tool is validated against mechanical testing results of melt electrospun tubes to show that a lesser winding angle provides improved mechanical response to uniaxial tension and compression. In addition, we show that melt electrospun tubes support the growth of three different cell types in vitro and are therefore promising scaffolds for tissue engineering applications. The online version of this article (doi:10.1007/s13758-011-0013-7) contains supplementary material, which is available to authorized users.