Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dur)
Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dur)
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DOI：
10.1038/srep37870
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发表时间：
2016-11-28
影响因子：
4.6
通讯作者：
Guo, Yu-Yuan
中科院分区：
文献类型：
--
作者：
An, Xing-Kui;Sun, Liang;Guo, Yu-Yuan
嗅觉受体被认为在昆虫寻找寄主、交配和产卵中起着核心作用。根据黄盲蝽成虫雌雄触角转录组，共鉴定出110个候选黄盲蝽气味受体（AlucOR），其中包括5个已标注的候选AlucOR。所有序列均经克隆和测序验证。组织表达谱分析显示，大部分AlucORs为触角高表达基因。qPCR检测进一步显示，触角中有40个AlucORs显著升高。1个AlucOR主要在雌性触角中表达，而9个AlucOR表现出雄性偏向的表达模式。此外，RPKM值和RT-qPCR分析均显示AlucOR83和AlucOR21在雄性触须中的丰度远高于雌性触须，表明它们在性别化学接受中的作用不同。系统发育分析表明，大多数alucor具有同源基因，并确定了5个alucor特异性分支。此外，在AlucORs的系统发育树上还发现了一个潜在的雄性性信息素受体亚枝。本研究结果将有助于对AlucORs基因的功能研究，从而为基于这些基因的新型害虫管理方法提供基础。
Olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. On the basis of male and female antennal transcriptome of adult Apolygus lucorum, a total of 110 candidate A. lucorum odorant receptors (AlucOR) were identified in this study including five previously annotated AlucORs. All the sequences were validated by cloning and sequencing. Tissue expression profiles analysis by RT-PCR indicated most AlucORs were antennal highly expressed genes. The qPCR measurements further revealed 40 AlucORs were significantly higher in the antennae. One AlucOR was primarily expressed in the female antennae, while nine AlucORs exhibited male-biased expression patterns. Additionally, both the RPKM value and RT-qPCR analysis showed AlucOR83 and AlucOR21 were much higher abundant in male antennae than in female antennae, suggesting their different roles in chemoreception of gender. Phylogenetic analysis of ORs from several Hemipteran species demonstrated that most AlucORs had orthologous genes, and five AlucOR-specific clades were defined. In addition, a sub-clade of potential male- based sex pheromone receptors were also identified in the phylogenetic tree of AlucORs. Our results will facilitate the functional studies of AlucORs, and thereby provide a foundation for novel pest management approaches based on these genes.