Distinct Growth Factor Families Are Recruited in Unique Spatiotemporal Domains during Long-Term Memory Formation in Aplysia californica.
Distinct Growth Factor Families Are Recruited in Unique Spatiotemporal Domains during Long-Term Memory Formation in Aplysia californica.
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DOI：
10.1016/j.neuron.2015.04.025
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发表时间：
2015-06-03
期刊：
影响因子：
16.2
通讯作者：
Carew TJ
中科院分区：
文献类型：
--
作者：
Kopec AM;Philips GT;Carew TJ
几种生长因子（GF）与长期记忆（LTM）有关，但没有一种GF能够支持记忆形成过程中发生的所有可塑性变化。由于GF参与高度收敛的信号级联，通常介导类似的功能结果，因此难以确定任何特定GF对LTM的相对贡献。为了探索这个问题，我们确定了不同的GF家族（通过TrkB和TGFβr-II进行信号传导）对Ablasia中LTM形成的独特贡献。我们证明了TrkB和TGFβr-II信号在两次试验训练期间在时间（分别通过试验1或2）和空间（在不同的亚细胞区室中）上的差异招募。这些GF独立调节MAPK活化，并协同调节基因表达。我们还表明，试验1 TrkB和试验2 TGFβr-II信号传导是LTM形成所必需的。这些数据支持这样的观点，即参与LTM形成的GF是复杂分子网络的相互作用的组成部分。
Several growth factors (GFs) have been implicated in long-term memory (LTM), but no single GF can support all the plastic changes that occur during memory formation. Because GFs engage highly convergent signaling cascades that often mediate similar functional outcomes, the relative contribution of any particular GF to LTM is difficult to ascertain. To explore this question, we determined the unique contribution of distinct GF families (signaling via TrkB and TGFβr-II) to LTM formation in Aplysia. We demonstrate that TrkB and TGFβr-II signaling are differentially recruited during Two-Trial training in both time (by Trial 1 or 2, respectively) and space (in distinct subcellular compartments). These GFs independently regulate MAPK activation, and synergistically regulate gene expression. We also show that Trial 1 TrkB and Trial 2 TGFβr-II signaling are required for LTM formation. These data support the view that GFs engaged in LTM formation are interactive components of a complex molecular network.