The impact of structural genomics: the first quindecennial.
The impact of structural genomics: the first quindecennial.
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DOI：
10.1007/s10969-016-9201-5
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Minor W
中科院分区：
文献类型：
--
作者：
Grabowski M;Niedzialkowska E;Zimmerman MD;Minor W
2000 年至 2015 年期间出现了高通量蛋白质结构测定方法。全球范围内成立的结构基因组学 (SG) 联盟的总资金约为 20 亿美元（以 2010 年美元计），开发了用于靶标选择、蛋白质生产、样品制备、结晶以及通过 X 射线晶体学和 NMR 确定结构的管道。这些努力确定了超过 13,500 个蛋白质结构，其中大部分来自独特的蛋白质家族，并增加了不断扩大的蛋白质宇宙的结构覆盖范围。 SG 项目为科学文献贡献了 4,400 多篇出版物。仅 NIH 资助的蛋白质结构计划 (PSI) 就已发表了 2,000 多份科学出版物，迄今为止已吸引了超过 93,000 次引用。处理高通量结构测定工作流程所需的软件和数据库开发导致结构质量更高，并提高了相关数据的完整性。有组织且可访问的数据对科学实验的可重复性产生积极影响。 SG 中心生成的大部分实验数据均可免费提供给社区，并已被各个研究领域的科学家所利用。 SG 项目创建、改进、简化和验证了许多蛋白质生产和结晶、数据收集和功能分析的方案，极大地有益于生物和生物医学研究。
The period 2000–2015 brought the advent of high-throughput approaches to protein structure determination. With the overall funding on the order of $2 billion (in 2010 dollars), the structural genomics (SG) consortia established worldwide have developed pipelines for target selection, protein production, sample preparation, crystallization, and structure determination by X-ray crystallography and NMR. These efforts resulted in the determination of over 13,500 protein structures, mostly from unique protein families, and increased the structural coverage of the expanding protein universe. SG programs contributed over 4,400 publications to the scientific literature. The NIH-funded Protein Structure Initiatives (PSI) alone have produced over 2,000 scientific publications, which to date have attracted more than 93,000 citations. Software and database developments that were necessary to handle high-throughput structure determination workflows have led to structures of better quality and improved integrity of the associated data. Organized and accessible data have a positive impact on the reproducibility of scientific experiments. Most of the experimental data generated by the SG centers are freely available to the community and has been utilized by scientists in various fields of research. SG projects have created, improved, streamlined, and validated many protocols for protein production and crystallization, data collection, and functional analysis, significantly benefiting biological and biomedical research.
影响因子：
12.3
作者：
Petsko GA
通讯作者：
Petsko GA