Role of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in the development of an acute neutrophil inflammatory response in mice
Role of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in the development of an acute neutrophil inflammatory response in mice
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DOI：
10.1182/blood.v88.10.3755.bloodjournal88103755
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发表时间：
1996-11-15
期刊：
影响因子：
20.3
通讯作者：
DiRago, L
中科院分区：
文献类型：
--
作者：
Metcalf, D;Robb, L;DiRago, L
将含有细菌的酪蛋白制剂腹膜内注射到小鼠中，由于从骨髓中选择性释放成熟细胞，在3小时内诱导嗜中性粒细胞的快速积聚。在此过程中，腹腔内粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）的浓度显著增加，但腹腔内注射这两种CSF均未诱导中性粒细胞的显著聚集，而G-CSF和酪蛋白共同注射未能增强中性粒细胞的反应。当将酪蛋白注射到编码GM-CSF、G-CSF或GM-CSF受体的β-共同链的基因失活的小鼠中时，通过典型的中性粒细胞渗出物的发展证实了CSF不参与中性粒细胞迁移。然而，预注射G-CSF增加了可用于迁移的骨髓中性粒细胞的数量，并导致腹膜腔中性粒细胞数量增加，酪蛋白注射液对注射酪蛋白或巯基乙酸盐的典型嗜酸性粒细胞炎症反应发生在GM-CSF -/-小鼠中，但不在β c -/-小鼠中，表明白细胞介素-5对于这种反应是必要的。(C)1996年，美国血液学会。
The intraperitoneal injection into mice of casein preparations containing bacteria induced a rapid accumulation of neutrophils within 3 hours due to selective release of mature cells from the bone marrow. Significant increases in the concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) occurred in the peritoneal cavity during the process, but the intraperitoneal injection of neither CSF induced a significant accumulation of neutrophils and the coinjection of G-CSF and casein failed to enhance the neutrophil response. The lack of involvement of either CSF in the neutrophil migration was confirmed by the development of typical neutrophil exudates when casein was injected into mice with inactivation of the genes encoding GM-CSF, G-CSF, or the beta-common chain of the GM-CSF receptor, However, preinjection of G-CSF increased the number of marrow neutrophils available for migration and did result in increased numbers of neutrophils in the peritoneal cavity after casein injection. Typical eosinophil inflammatory responses to the injection of casein or thioglycollate occurred in GM-CSF -/- mice but not in beta c -/- mice, suggesting that interleukin-5 was necessary for this response. (C) 1996 by The American Society of Hematology.