OPA1 promotes pH flashes that spread between contiguous mitochondria without matrix protein exchange.
OPA1 promotes pH flashes that spread between contiguous mitochondria without matrix protein exchange.
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DOI：
10.1038/emboj.2013.124
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发表时间：
2013-07-03
期刊：
影响因子：
11.4
通讯作者：
Demaurex, Nicolas
中科院分区：
文献类型：
--
作者：
Santo-Domingo, Jaime;Giacomello, Marta;Poburko, Damon;Scorrano, Luca;Demaurex, Nicolas
与线粒体膜电位波动相关的线粒体闪光的化学性质和功能意义尚不清楚。使用对超氧化物不敏感的比例pH探针，我们发现闪光反映了在无离子溶液中渗透的细胞中持续存在的~ 0.4 pH单位的基质碱化瞬变，并且可以通过施加线粒体去极化引起。消融促融合蛋白Optic atrophy 1特异性地消除了pH闪烁并减少了基质光激活GFP （paGFP）的繁殖。前裂变动力蛋白相关蛋白1的消融或失效极大地增强了相邻线粒体之间的闪速繁殖，但略微增加了paGFP基质的扩散，表明闪速在没有基质内容交换的情况下繁殖。pH闪烁与同步去极化和超极化事件有关，这些事件促进了并置线粒体的膜电位平衡。我们认为闪光是由两个具有不同膜电位的相邻线粒体之间的融合孔打开触发的能量守恒事件，在没有基质融合的情况下传播，以平衡连接线粒体的能量状态。
The chemical nature and functional significance of mitochondrial flashes associated with fluctuations in mitochondrial membrane potential is unclear. Using a ratiometric pH probe insensitive to superoxide, we show that flashes reflect matrix alkalinization transients of ∼0.4 pH units that persist in cells permeabilized in ion-free solutions and can be evoked by imposed mitochondrial depolarization. Ablation of the pro-fusion protein Optic atrophy 1 specifically abrogated pH flashes and reduced the propagation of matrix photoactivated GFP (paGFP). Ablation or invalidation of the pro-fission Dynamin-related protein 1 greatly enhanced flash propagation between contiguous mitochondria but marginally increased paGFP matrix diffusion, indicating that flashes propagate without matrix content exchange. The pH flashes were associated with synchronous depolarization and hyperpolarization events that promoted the membrane potential equilibration of juxtaposed mitochondria. We propose that flashes are energy conservation events triggered by the opening of a fusion pore between two contiguous mitochondria of different membrane potentials, propagating without matrix fusion to equilibrate the energetic state of connected mitochondria.
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影响因子：
21.3
作者：
通讯作者：
--
影响因子：
4
作者：
De Giorgi, F;Lartigue, L;Ichas, F
通讯作者：
Ichas, F
影响因子：
6.2
作者：
Azarias, Guillaume;Van de Ville, Dimitri;Chatton, Jean-Yves
通讯作者：
Chatton, Jean-Yves
影响因子：
3.5
作者：
Ban T;Heymann JA;Song Z;Hinshaw JE;Chan DC
通讯作者：
Chan DC
DOI：
10.1111/j.1432-1033.1974.tb03899.x
发表时间：
1974-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
NICHOLLS, DG
通讯作者：
NICHOLLS, DG