Enzymatic Cascades for Efficient Biotransformation of Racemic Lactate Derived from Corn Steep Water
Enzymatic Cascades for Efficient Biotransformation of Racemic Lactate Derived from Corn Steep Water
复制标题
用于高效生物转化玉米浸泡水中外消旋乳酸的酶级联
DOI：
10.1021/acssuschemeng.7b00136
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发表时间：
2017-04-01
影响因子：
8.4
通讯作者：
Gao, Chao
中科院分区：
文献类型：
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作者：
Li, Zhong;Zhang, Manman;Gao, Chao
玉米淀粉行业产生大量的玉米浸泡水，导致高有机废物负荷。以较低的成本从玉米碱水中分离出乳酸，为玉米碱水的增值利用提供了支持。然而，玉米浸泡水中乳酸的外消旋特性限制了这一有前景的工艺的发展。本研究以氧化葡萄糖杆菌的邻乳酸氧化酶((D)-LOX)、链球菌的L-乳酸氧化酶((L)-LOX)、运动发酵单胞菌的丙酮酸脱羧酶和牛肝的过氧化氢酶为原料，合成了一个包括不同级联酶的体外酶系，用于从玉米碱水中提取乳酸，以生产有价值的平台化合物。丙酮酸是一种重要的中间体，在丙酮酸脱羧酶的优化浓度下，丙酮酸被高产率地转化为C2(乙醛)和C4(乙酮)平台化合物。该体外酶体系不仅为光学乳酸盐及其衍生物的生产提供了新的技术平台，而且为玉米淀粉产业的可持续发展提供了支持。
The corn starch industry produces a large amount of corn steep water, leading to high organic waste load. Lactate could be separated from corn steep water at a low cost, which supports the value-added utilization of corn steep water. However, the racemic characteristic of lactate from corn steep water restricts the development of this promising process. In this study, using o-lactate oxidase ((D)-LOX) from Gluconobacter oxydans, L-lactate oxidase ((L)-LOX) from Pediococcus sp., pyruvate decarboxylase from Zymomonas mobilis, and catalase from bovine liver, we synthesized an in vitro enzymatic system, including different enzymatic cascades, for the production of valuable platform chemicals from lactate separated from the corn steep water. Pyruvate was produced as an important intermediate and further converted into C2 (acetaldehyde) and C4 (acetoin) platform chemicals at high yields using optimized concentrations of pyruvate decarboxylase. The in vitro enzymatic system not only provides a novel technology platform for the production of optical lactate and lactate derivatives but also supports the sustainable development of corn starch industry.