Prevalence and mechanisms of macrolide resistance in clinical isolates of group A streptococci from Ontario, Canada
Prevalence and mechanisms of macrolide resistance in clinical isolates of group A streptococci from Ontario, Canada
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DOI：
10.1128/aac.43.9.2144
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发表时间：
1999-09-01
影响因子：
4.9
通讯作者：
Low, DE
中科院分区：
文献类型：
--
作者：
De Azavedo, JCS;Yeung, RH;Low, DE
1997年，通过一个私人实验室共收集了3 205个A组链球菌分离株，该实验室为安大略省南部的社区医生提供服务，该实验室的人口基数为600万。纸片扩散法和肉汤微量稀释法检测67株（2.1%）菌株对红霉素不敏感。其中，47例（70%）对克林霉素敏感，经PCR检测发现具有mef基因。其余20株中，分别有18株和2株对克林霉素产生诱导抗性和组成抗性。PCR结果显示，其中19株具有ermTR基因，1株具有ermB基因。16株（24%）红霉素耐药菌株对四环素也耐药，对青霉素和氯霉素均敏感。
A total of 3,205 group A streptoccal isolates were collected in 1997 through a private laboratory which serves community physicians in southern Ontario and which represents a population base of 6 million people. Nonsusceptibility to erythromycin was detected for 67 (2.1%) isolates both by disk diffusion and by broth microdilution. Of these, 47 (70%) were susceptible to clindamycin and were found by PCR to possess the mef gene. Of the other 20 strains, 18 and 2 showed inducible and constitutive resistance, respectively, to clindamycin. Nineteen of these strains were show by PCR to possess the ermTR gene, and a single constitutively resistant strain harbored an ermB gene. Sixteen (24%) erythromycin-resistant strains were also resistant to tetracycline, All were susceptible to penicillin and chloramphenicol.