In vivo cytometry: A spectrum of possibilites
In vivo cytometry: A spectrum of possibilites
复制标题
DOI：
10.1002/cyto.a.20220
复制
发表时间：
2006-03-01
期刊：
影响因子：
3.7
通讯作者：
Farkas, DL
中科院分区：
文献类型：
--
作者：
Chung, A;Karlan, S;Farkas, DL
Background: We investigate whether optical imaging can reliably detect abnormalities in tissue, in a range of specimens (live cells in vitro; fixed, fresh ex-vivo and in Vivo tissue), without the use of added contrast agents, and review our promising spectral methods for achieving quantitative, real-time, high resolution intrasurgical optical diagnostics.Methods: We use reflectance, fluorescence, two-photon, and Mic scattering imaging, performed with instrumentation we developed or modified, to detect intrinsic tissue signatures. Emphasis is on spectral/hyperspectral imaging approaches allowing the equivalent of in vivo pathology.Results: With experimental focus on unstained specimens, we demonstrate the ability to segment tissue images for cancer detection. Spectral reflectance imaging, Coupled with advanced analysis, typically yields 90% specificity and sensitivity. Autofluorescence is also shown to be diagnostically useful, with lymph nodes results highlighted here. Elastic scattering hyperspectral imaging endoscopy, using a new instrument we designed and built, shows promise in bronchoscopic detection of dysplasia and early cancer in patients.Conclusions: The results demonstrate that advanced optical imaging can detect and localize cellular signatures of cancer in real-time, in vivo, without the use of contrast agents, in animals and humans. This is an important step towards tight spatio-temporal coupling between such detection and clinical intervention. (c) 2006 international Society for Analytical Cytology.
