Basic fibroblast growth factor experimentally induced choroidal angiogenesis in the minipig.
Basic fibroblast growth factor experimentally induced choroidal angiogenesis in the minipig.
复制标题
碱性成纤维细胞生长因子通过实验诱导小型猪脉络膜血管生成。
作者：
Gisèle Soubrane;S. Y. Cohen;Thierry Delayre;Jacqueline Tassin;Marie;G. Coscas;Yves Courtois;J. Jeanny
碱性成纤维细胞生长因子是一种可溶性的有丝分裂原，已从包括视网膜在内的多种器官中分离纯化。体内血管生成活性已通过多种检测方法得到证实。用渗透性微泵灌流重组人碱性成纤维细胞生长因子，建立小型猪脉络膜新生血管模型。用免疫组织化学和放射自显影技术对正常猪视网膜内源性bFGF及其受体进行了定位。外源性碱性成纤维细胞生长因子在脉络膜上腔内沿导管最后3 mm处诱导出排列良好的新生血管。这种新生血管并没有穿透正常的Bruch膜。血管细胞(用von Willebrand因子抗体染色鉴定)从眼内导管近端到眼内导管末端的数量和表面均增加。在用磷酸盐缓冲盐水灌流的眼(对照组)，但在bFGF灌流的眼中，发生了炎症反应(由巨噬细胞特异性抗体鉴定)。这些结果表明，通过在脉络膜上腔内灌流过量的碱性成纤维细胞生长因子，可以在没有炎症反应的情况下实现脉络膜血管生成。
Basic fibroblast growth factor (bFGF), a soluble mitogen, has been isolated and purified from various organs, including the retina. In vivo angiogenic activity of bFGF has been demonstrated with several assays. An experimental model of choroidal neovascularization was developed in the mini pig by perfusion of recombinant human bFGF through an osmotic minipump. Endogenous bFGF and bFGF receptors were localized in the normal pig retina by immunohistochemistry and autoradiography after binding. The perfusion of exogenous bFGF induced well-organized new vessels along the last 3 mm of the catheter in the suprachoroidal space. This neovascularization did not penetrate the normal Bruch's membrane. Vascular cells (identified by von Willebrand factor antibody staining) increased in number and in surface from the proximal part to the end of the intraocular catheter in all bFGF perfused eyes. In eyes perfused with phosphate buffered saline (controls), but not in the bFGF perfused eyes, an inflammatory response occurred (identified by a macrophage specific antibody). These results demonstrate that choroidal angiogenesis can be achieved without an inflammatory response by perfusing an excess of bFGF in the suprachoroidal space.
培养的人视网膜色素上皮细胞表达碱性成纤维细胞生长因子及其受体。
影响因子：
2
作者：
Sternfeld,MD;Robertson,JE;Shipley,GD;Tsai,J;Rosenbaum,JT
通讯作者：
Rosenbaum,JT
小仓鼠肾细胞成纤维细胞生长因子受体的鉴定和部分表征。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Neufeld,G;Gospodarowicz,D
通讯作者：
Gospodarowicz,D
DOI：
10.1016/0006-291x(87)90292-0
发表时间：
1987-01-30
影响因子：
3.1
作者：
BAIRD, A;LING, N
通讯作者：
LING, N