Catalpa seed oil rich in 9t,11t,13c-conjugated linolenic acid suppresses the development of colonic aberrant crypt foci induced by azoxymethane in rats.
Catalpa seed oil rich in 9t,11t,13c-conjugated linolenic acid suppresses the development of colonic aberrant crypt foci induced by azoxymethane in rats.
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富含 9t、11t、13c 共轭亚麻酸的梓籽油可抑制氧化偶氮甲烷诱导的大鼠结肠异常隐窝病灶的发展。
DOI：
10.3892/or.16.5.989
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发表时间：
2006
期刊：
影响因子：
4.2
通讯作者：
Takuji Tanaka
中科院分区：
文献类型：
--
作者：
Rikako Suzuki;Y. Yasui;H. Kohno;S. Miyamoto;M. Hosokawa;K. Miyashita;Takuji Tanaka
梓籽油（Catalpa ovata）是一种含有大量9-反式、11-反式、13-顺式共轭亚麻酸的独特油脂。在本研究中，我们调查是否饮食管理与CPO影响的发展氧化偶氮甲烷（AOM）诱导的结肠异常隐窝灶（ACF）在雄性F344大鼠，以阐明其可能的癌症化学预防效率。观察CPO对肝组织和结肠黏膜脂肪酸组成、血清总胆固醇和甘油三酯水平及结肠黏膜环氧合酶（考克斯）-2 mRNA表达的影响。同时，采用免疫组化法检测结肠黏膜细胞增殖活性和凋亡指数。动物每周两次皮下注射AOM（20 mg/kg体重）。他们还接受了含有0.01%，0.1%或1%CPO的实验饮食4周，从AOM首次给药前一周开始。AOM暴露在研究结束时（第4周）产生大量ACF（99+/-28）。日粮中添加CPO可减少ACF数量（AOM +0.01%CPO，32+/-11，P <0.001; AOM +0.1%CPO，35+/-18，P <0.001; AOM +1%CPO，18+/-10，P <0.001）。在喂食含CPO饲料的大鼠的肝组织和结肠粘膜中检测到9 t，11 t-共轭亚油酸。此外，饮食管理与CPO降低血清甘油三酯水平和考克斯-2 mRNA在结肠粘膜中的表达。AOM和1%CPO处理组大鼠结肠粘膜细胞增殖和凋亡指数与AOM单独处理组相比有显著性差异。这些结果表明，可能的化学预防活性的CPO在结肠癌发生的早期阶段。
Catalpa (Catalpa ovata) seed oil (CPO) is a unique oil that contains a high amount of 9trans,11trans,13cis-conjugated linolenic acid. In the present study, we investigated whether dietary administration with CPO affects the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in male F344 rats to elucidate its possible cancer chemopreventive efficiency. Also, the effect of CPO on the fatty acid composition of liver tissue and colonic mucosa, the serum levels of total cholesterol and triglyceride, and the mRNA expression of cyclooxygenase (COX)-2 in the colonic mucosa were measured. In addition, the cell proliferation activity and apoptotic index in the colonic mucosa were estimated immunohistochemically. Animals were given two weekly subcutaneous injections of AOM (20 mg/kg body weight). They also received the experimental diet containing 0.01%, 0.1% or 1% CPO for 4 weeks, starting one week before the first dosing of AOM. AOM exposure produced a substantial number of ACF (99+/-28) at the end of the study (week 4). Dietary administration of CPO reduced the number of ACF (AOM + 0.01% CPO, 32+/-11, P<0.001; AOM + 0.1% CPO, 35+/-18, P<0.001; AOM + 1% CPO, 18+/-10, P<0.001). 9t,11t-conjugated linoleic acid was detected in the liver tissue and colonic mucosa of rats fed the CPO-containing diet. Additionally, dietary administration with CPO decreased the serum triglyceride level and the expression of COX-2 mRNA in the colonic mucosa. The indices of cell proliferation and apoptosis in the colonic mucosa of rats treated with AOM and 1% CPO have significant differences when compared with the AOM alone group. These findings suggest the possible chemopreventive activity of CPO in the early phase of colon carcinogenesis.
影响因子：
11.2
作者：
Y. Ha;J. Storkson;M. Pariza
通讯作者：
Y. Ha;J. Storkson;M. Pariza