MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein
MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein
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DOI：
10.1159/000059424
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发表时间：
2002-05-01
影响因子：
11
通讯作者：
Roeder, RG
中科院分区：
文献类型：
--
作者：
Estable, MC;Naghavi, MH;Roeder, RG
正转录延伸因子b（Positive transcription elongation factor-b，P-TEFb）含有CDK 9和细胞周期蛋白T-1。从表达表位标记的CDK 9的稳定转染的细胞系中亲和纯化P-TEFb，并对似乎特异性结合的蛋白质进行测序。除了CDK 9、先前鉴定的细胞周期蛋白T（包括T-1、T-2A和T-2B）HSP 90和CDC 37的同种型之外，该分析鉴定了一种新的蛋白质，称为MCEF。其同源cDNA的克隆显示，MCEF是参与急性淋巴细胞白血病的转录因子AF 4家族的最新成员。MCEF RNA在所有检测的人体组织中表达，抗重组MCEF特异性抗血清免疫沉淀P-TEFb。MCEF的异位表达不激活HIV-1复制，并且MCEF与启动子的连接不激活转录。版权所有（C）2002国家科学理事会，中华民国和S. Karger AG，巴塞尔。
Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T-1. P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T-1, T-2A and T-2B) HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF Specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription. Copyright (C) 2002 National Science Council, ROC and S. Karger AG, Basel.