Immune distribution and localization of phosphoantigen-specific Vγ2Vδ2 T cells in lymphoid and nonlymphoid tissues in Mycobacterium tuberculosis infection
Immune distribution and localization of phosphoantigen-specific Vγ2Vδ2 T cells in lymphoid and nonlymphoid tissues in Mycobacterium tuberculosis infection
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DOI：
10.1128/iai.01008-07
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Chen, Zheng W.
中科院分区：
文献类型：
--
作者：
Huang, Dan;Shen, Yun;Chen, Zheng W.
在人类感染过程中，抗原特异性T细胞在组织/器官粘膜界面的免疫分布和定位知之甚少。在这项研究中，我们利用结核分枝杆菌感染的猕猴模型来评估磷酸化抗原特异性V γ 2 V δ 2 T细胞的组织分布、解剖学定位以及在严重肺结核进展中淋巴和非淋巴器官/组织中是否存在结核（TB）病变的相关性。肺结核的进展。结核病感染产生了不同的V γ 2 V δ 2 T细胞分布模式，这些细胞在肺、支气管淋巴结、脾和远端非淋巴器官中显著积聚，但在血液中没有。组织中V γ 2 V δ 2 T细胞数量的增加与M。结核感染，但独立于结核病病变的严重程度。在具有明显TB病变的肺中，V γ 2 V δ 2 T细胞存在于TB肉芽肿内。在胸外器官中，V γ 2 V δ 2 T细胞定位于非淋巴组织的间质区室中，尽管没有可检测到的TB病变，但仍存在间质定位。最后，组织中积累的V γ 2 V δ 2 T细胞似乎具有细胞因子产生功能，因为在肉芽肿内存在的γ δ T细胞中可检测到颗粒酶B。因此，克隆扩增的V γ 2 V δ 2 T细胞似乎经历了跨内皮迁移、间质定位和肉芽肿浸润作为对M的免疫应答。肺结核感染。
Little is known about the immune distribution and localization of antigen-specific T cells in mucosal interfaces of tissues/organs during infection of humans. In this study, we made use of a macaque model of Mycobacterium tuberculosis infection to assess phosphoantigen-specific V gamma 2V delta 2 T cells regarding their tissue distribution, anatomical localization, and correlation with the presence or absence of tuberculosis (TB) lesions in lymphoid and nonlymphoid organs/tissues in the progression of severe pulmonary TB. Progression of pulmonary M. tuberculosis infection generated diverse distribution patterns of V gamma 2V delta 2 T cells, with remarkable accumulation of these cells in lungs, bronchial lymph nodes, spleens, and remote nonlymphoid organs but not in blood. Increased numbers of V gamma 2V delta 2 T cells in tissues were associated with M. tuberculosis infection but were independent of the severity of TB lesions. In lungs with apparent TB lesions, V gamma 2V delta 2 T cells were present within TB granulomas. In extrathoracic organs, V gamma 2V delta 2 T cells were localized in the interstitial compartment of nonlymphoid tissues, and the interstitial localization was present despite the absence of detectable TB lesions. Finally, V gamma 2V delta 2 T cells accumulated in tissues appeared to possess cytokine production function, since granzyme B was detectable in the gamma delta T cells present within granulomas. Thus, clonally expanded V gamma 2V delta 2 T cells appeared to undergo trans-endothelial migration, interstitial localization, and granuloma infiltration as immune responses to M. tuberculosis infection.