MORPHOLOGICAL-STUDY OF THE EFFECTS OF INTRANASAL ZINC-SULFATE IRRIGATION ON THE MOUSE OLFACTORY EPITHELIUM AND OLFACTORY-BULB
MORPHOLOGICAL-STUDY OF THE EFFECTS OF INTRANASAL ZINC-SULFATE IRRIGATION ON THE MOUSE OLFACTORY EPITHELIUM AND OLFACTORY-BULB
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DOI：
10.1002/jemt.1070240302
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发表时间：
1993-02-15
影响因子：
2.5
通讯作者：
BURD, GD
中科院分区：
文献类型：
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作者：
BURD, GD
研究了鼻内硫酸锌（ZnSO4）灌洗对短存活时间（早1天）和长存活时间（长达593天）小鼠嗅上皮和嗅球形态的影响。与之前的几项研究一样，嗅觉上皮在ZnSO4处理后几天内被完全破坏。在2-4天内，鼻中隔和鼻甲被一个新的立方体上皮覆盖，其细胞与通常在嗅觉上皮中看到的任何细胞都有明显的不同。经过几个月的时间，许多动物的嗅上皮的小区域慢慢地再生了。1 ~ 25天（反应期）嗅球的超微结构变化表现为嗅轴突和轴突末梢变性，星形胶质细胞突肥大，吞噬细胞增生或外渗。鼻腔灌洗ZnSO4后25 d，反应性胶质突和吞噬细胞数量恢复正常。在一些存活150天或更长时间的小鼠中，再生的嗅轴突在嗅球的小区域进行了再神经支配。这些新的嗅觉轴突仅支配浅层肾小球或深层肾小球的外层，但它们与二尖瓣/簇状细胞和肾小球周围细胞形成突触接触，与对照动物没有区别。这些发现得到了用h -3氨基酸进行的追踪实验和行为分析的支持。综上所述，本研究中观察到的嗅球超微结构变化与嗅觉上皮或神经手术病变后观察到的变化无显著差异。然而，嗅球从未完全恢复；肾小球仍然萎缩（尽管有正常的树突-树突突触连接），嗅觉轴突再神经化极少。
The effects of intranasal zinc sulfate (ZnSO4) irrigation on the morphology of the olfactory epithelium and olfactory bulb were studied in mice with short survival times (as early as 1 day) and with long survival times (up to 593 days) after the irrigation procedure. As in several previous studies, the olfactory epithelium was completely destroyed within a few days after the ZnSO4 treatment. Within 2-4 days, the septum and turbinates were covered by a new, cuboidal epithelium, the cells of which differed significantly from any cells normally seen in the olfactory epithelium. Slowly, over several months, small areas of the olfactory epithelium regenerated in many of the animals.The ultrastructural changes occurring in the olfactory bulb from 1 to 25 days (the reactive stage) were characterized by degenerating olfactory axons and axon terminals, hypertrophy of astroglial cell processes, and proliferation of or extravasation by phagocytic cells. By 25 days after intranasal ZnSO4 irrigation, the number of reactive glial processes and phagocytic cells returned to normal. In some mice with survival times of 150 days or longer, there was reinnervation of small areas of the olfactory bulb by regenerated olfactory axons. These new olfactory axons innervated only superficial glomeruli or the outer portions of deeper glomeruli, but they formed synaptic contacts with mitral/tufted cells and periglomerular cells that did not differ from control animals. These findings were supported by tract-tracing experiments with H-3-amino acids and by behavioral analysis.In summary, the ultrastructural changes observed in the olfactory bulb in this study were not significantly different from those observed after surgical lesions of the olfactory epithelium or nerve. The olfactory bulb, however, never fully recovered; glomeruli remained shrunken (though with normal dendro-dendritic synaptic connections), and there was minimal olfactory axon reinnervation.