Observation of the interaction between avidin and iminobiotin using a graphene FET on a SiC substrate
Observation of the interaction between avidin and iminobiotin using a graphene FET on a SiC substrate
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DOI：
10.7567/1347-4065/ab0544
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发表时间：
2019-05
影响因子：
1.5
通讯作者：
Y. Taniguchi;T. Miki;Y. Ohno;M. Nagase;Y. Arakawa;M. Yasuzawa
中科院分区：
文献类型：
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作者：
Y. Taniguchi;T. Miki;Y. Ohno;M. Nagase;Y. Arakawa;M. Yasuzawa
在碳化硅衬底上，功能化外延石墨烯场效应管(GFET)可以检测到精细的化学变化，有望具有单晶石墨烯薄膜的本征传感特性。制备了亚胺生物素(IB)修饰的GFET，用于测量亲和素与IB之间的相互作用。在pH为9的溶液中，IB与亲和素发生强烈的相互作用，而在较低的pH时，这种相互作用变得很弱。在pH=7和pH=9时，亲和素的电学性质随亲和素浓度的增加而变化，可用吸附等温线进行拟合。然而，在pH为4时观察到了几乎恒定的电学特性。这些pH依赖关系清楚地表明，在碳化硅衬底上外延石墨烯薄膜的改性GFET可以作为环境传感器来检测微小的化学反应。
Fine chemical changes were electrically detected by functionalized epitaxial graphene field-effect transistors (GFET) on SiC substrates, which were expected to have the intrinsic sensing characteristics of a single-crystal graphene film. An iminobiotin (IB)-modified GFET was fabricated to measure the interaction between avidin and IB. IB interacts strongly with avidin in a solution at pH 9, while the interaction becomes weak at a lower pH. The electrical characteristics shifted with increasing concentration of avidin at pH 7 and pH 9, and could be fitted by the adsorption isotherm. However, almost constant electrical characteristics were observed at pH 4. These pH dependences clearly showed that a modified GFET with an epitaxial graphene film on a SiC substrate can act as an environmental sensor for small chemical reactions.