Facilitation of iontophoretic drug delivery through intact and caries-affected dentine
Facilitation of iontophoretic drug delivery through intact and caries-affected dentine
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DOI：
10.1046/j.1365-2591.2003.00712.x
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发表时间：
2003-10-01
影响因子：
5
通讯作者：
Suda, H
中科院分区：
文献类型：
--
作者：
Puapichartdumrong, P;Ikeda, H;Suda, H
目的探讨离子导入对牙本质完整和龋损给药的促进作用。方法从新鲜拔除的人第三磨牙制备48个完整的牙本质或患龋牙本质膜。分别在实验前后进行了导水率的测量。使用分隔室装置评估有无离子导入(0.05 mA，10分钟)的药物扩散。牙釉质侧充填甲硝唑(MN)、水杨酸钠(SS)或萘普生钠(NA)，牙髓侧充填磷酸盐缓冲盐水(PBS)，加压15cmH(2)O。1h后取材，用分光光度计法测定牙髓侧液的药物浓度。然后测量每个牙本质盘的电阻抗。结果离子导入、龋病引起的牙本质改变和所用药物对药物扩散均有显著影响(P<；0.05)。所有药物在患龋牙本质中的扩散均显著低于在完整牙本质中的扩散(独立t检验，P<；0.05)。此外，离子导入促进了所有药物通过完整的和受龋齿影响的牙本质的扩散。SS的药物扩散显著高于MN和NA(One-way ANOVA，P<；0.05)，不依赖于离子载体。结论龋病的存在可能抑制药物通过牙本质向牙髓的扩散。然而，离子导入可以通过完整的牙本质和受龋齿影响的牙本质增强电离药物的输送。
Aim To investigate the facilitative role of iontophoresis in drug delivery through intact and caries-affected dentine into the pulp.Methodology Forty-eight intact dentine or caries-affected dentine discs were prepared from freshly extracted human third molars. The hydraulic conductance was measured before and after the experiments. Drug diffusion with and without iontophoresis (0.05 mA, 10 min) was evaluated using a split-chamber device. The enamel side chamber was filled with metronidazole (MN), sodium salicylate (SS) or naproxen sodium (NA), while the pulpal side was circulated with phosphate buffered saline (PBS) and pressurized (15 cmH(2)O). Samples were collected after 1 h. Drug concentrations of the pulpal side solution were determined using a spectrophotometer. Then, electrical impedance of each dentine disc was measured. Finally, the dentine disc surfaces were observed by scanning electron microscopy.Results Drug diffusion was significantly influenced by iontophoresis, caries-induced changes in dentine and the drugs used (three-way ANOVA, P < 0.05). The diffusion of all the drugs through caries-affected dentine was significantly less than that through intact dentine (independent t-test, P < 0.05). Also, iontophoresis facilitated the diffusion of all the drugs through intact and caries-affected dentine. The drug diffusion of SS was significantly higher than MN and NA (one-way ANOVA, P < 0.05), independent of iontophoresis.Conclusions Presence of dental caries may inhibit drug diffusion through dentine into the pulp. However, iontophoresis could enhance the delivery of ionized drugs through both intact and caries-affected dentine.