Comparison of physiological and methylational changes in resynthesized Brassica napus and diploid progenitors under drought stress
Comparison of physiological and methylational changes in resynthesized Brassica napus and diploid progenitors under drought stress
复制标题
干旱胁迫下再合成甘蓝型油菜和二倍体祖细胞的生理和甲基化变化的比较
DOI：
10.1007/s11738-019-2837-6
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发表时间：
2019
影响因子：
2.6
通讯作者：
Wang Youping
中科院分区：
文献类型：
--
作者：
Jiang Jinjin;Yuan Yi;Zhu Shuang;Fang Tingting;Ran Liping;Wu Jian;Wang Youping
甘蓝型油菜是一种具有一定研究价值和经济价值的多倍体。再合成B。甘蓝型油菜二倍体B。rapa和B.由于B的遗传背景有限，羽衣甘蓝对于芸苔属研究至关重要。油菜。考虑到多倍体比相应的二倍体具有更好的农艺性状和抗性，我们研究了B多倍体化后的耐旱性。揭示了多倍体与二倍体的表观遗传差异。干旱胁迫后，B.芜菁和人工合成杂种一代（F1）比B萎蔫。oleracea和F2-F4代。干旱胁迫后，F1的相对含水量和持水率较好。F1的气孔密度低于F2，气孔大小显著小于F3。不同世代的B的生理指标存在差异。甘蓝型油菜和二倍体亲本的这些参数在杂种中大多高于B。rapa低于B。甘蓝。但F3和F4的过氧化物酶活性显著高于双亲，丙二醛含量低于双亲，表明F3和F4可能比其他世代更能适应氧化胁迫。与F1相比，F2和F3的DNA甲基化水平降低，然后在F4中升高。甲基化敏感扩增多态性分析表明，干旱胁迫后，DNA甲基化和去甲基化广泛发生。甲基化和去甲基化水平为F1 F4 B。甘蓝F2 F3 B. rapa和B.芜菁F4 F3 F2 B. oleracea F1。干旱胁迫下的表观遗传学变化可能与B期间不同的胁迫耐受性有关。甘蓝型油菜多倍体化
Brassica napus is a polyploid of certain research and economical value. Resynthesizing B. napus with diploid B. rapa and B. oleracea is essential for Brassica research because of the limited genetic background of B. napus. Considering that polyploids possess better agronomic traits and resistance compared with the corresponding diploids, we investigated drought tolerance after polyploidization of B. napus and revealed the epigenetic differences between polyploids and diploids. After drought stress, B. rapa and first-generation of synthesized hybrids (F1) were more wilted than B. oleracea and F2–F4 generations. However, the relative water content and water retention in F1 were better than others after drought stress. The increased number of partially opened and closed stomata in F1 was not as significant as that in F2 and F3, but stomata density in F1 was lower than F2, and the stomatal size in F1 was significantly reduced than F3. Physiological parameters varied among different generations of B. napus and diploid parents, and most of these parameters in hybrids were higher than B. rapa and lower than B. oleracea. However, the peroxidase activity in F3 and F4 was significantly higher than both parents, and the malondialdehyde content in F3 and F4 was lower than both parents, indicating that F3 and F4 might be more adaptive to oxidative stresses than other generations. DNA methylation level was decreased in F2 and F3 compared with F1, and then increased in F4. Methylation-sensitive amplified polymorphism analysis revealed that DNA methylation and demethylation broadly happened after drought stress. The methylation and demethylation level was F1 > F4 > B. oleracea > F2 > F3 > B. rapa and B. rapa > F4 > F3 > F2 > B. oleracea > F1, respectively. The epigenetic changes under drought stress might be related to the different stress tolerances during B. napus polyploidization.