Bacterial diversity losses: A potential extracellular driving mechanism involving the molecular ecological function of hydrophobic polycyclic aromatic hydrocarbons.
Bacterial diversity losses: A potential extracellular driving mechanism involving the molecular ecological function of hydrophobic polycyclic aromatic hydrocarbons.
复制标题
细菌多样性丧失：涉及疏水性多环芳烃分子生态功能的潜在细胞外驱动机制
DOI：
10.1016/j.btre.2014.11.002
复制
发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Zhou Q
中科院分区：
文献类型：
--
作者：
Hu X;Kang F;Gao Y;Zhou Q
DNA 转化对于水平基因转移 (HGT) 至关重要。暴露于疏水性多环芳烃（PAHs）的裸质粒的低效转化降低了基因转移水平，可能与目前细菌多样性的丧失有关。 PAH 通过色散力和 PAH 与碱基之间的 π-π 重叠，对裸露 DNA 具有很强的亲和力。 PAH 和碱基之间的这些非共价相互作用降低了质粒向细菌受体的转化效率。同时，这些低效率的质粒转化受到环境中 Ca2+ 等离子的控制，反过来，0.5 mmol L−1 Ca2+ 的存在将效率从 3.2（菲）、3.5（芘）分别恢复到约 4.45 和 4.75。 Ca2+与DNA中的-POO--基团结合形成强电价键，削弱DNA对PAHs的分子作用，进而促进暴露于PAHs的基因转移。
The DNA transformation is vital to the horizontal gene transfer (HGT). The low-efficiency transformation of bare plasmid exposed to hydrophobic polycyclic aromatic hydrocarbons (PAHs) decreases the gene transfer level, and is possibly related to the loss of bacterial diversity at present. PAHs have great affinity for bare DNA through dispersion force and π–π overlap between PAHs and bases. These noncovalent interactions between PAHs and bases reduced the transformational efficiency of plasmid into bacterial recipients. Meanwhile these low-efficiency transformations for plasmid are controlled by the ions like Ca2+ in environment, in turn, presence of 0.5 mmol L−1 Ca2+ recovered the efficiency from 3.2 (phenanthrene), 3.5 (pyrene) to about 4.45 and 4.75, respectively. The combination of Ca2+ with the —POO−— groups in DNA forms strong electrovalent bonds, weakening the molecular effect of DNA on PAHs and in turn promoting the gene transfer exposed to PAHs.