Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family.
Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family.
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DOI：
10.1016/s0021-9258(18)35946-5
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发表时间：
1992-11
期刊：
影响因子：
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通讯作者：
Stephen Lee;A. Francoeur;Shan Liu;E. Wang
中科院分区：
文献类型：
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作者：
Stephen Lee;A. Francoeur;Shan Liu;E. Wang
延伸因子 1 α (EF-1 α) 是一种普遍存在的蛋白质，在 mRNA 翻译过程中发挥肽延伸作用。我们之前报道过分离出一种与他汀类药物（一种非增殖特异性蛋白）抗原相关的大鼠 S1 蛋白；该 S1 基因与 EF-1 α 具有高度同源性。基于 S1 和 EF-1 α mRNA 3' 非编码区的差异，我们构建了针对这两个基因的特异性核糖核酸探针。 Northern 分析和 RNase 保护测定表明，S1 mRNA 仅存在于大脑、心脏和肌肉中，而 EF-1 α mRNA 已在迄今为止调查的所有组织中检测到。在小鼠中观察到相同的组织特异性，表明 S1 表达在这两种哺乳动物之间是保守的。 S1 转录物在大脑胚胎发生后期（第 20 天）被检测到，但含量低于 3 个月大的成人大脑。我们发现，S1 和 EF-1 α 转录物的相对水平及其各自的组织丰度在衰老过程中保持不变。 S1的功能尚不清楚；但这些结果表明，它可能参与组织中细胞（即神经元和肌细胞）永久锁定在不增殖状态的蛋白质合成的特定控制机制。
Elongation factor-1 alpha (EF-1 alpha) is an ubiquitous protein that functions in peptide elongation during mRNA translation. We previously reported the isolation of a rat S1 protein that is antigenically related to statin, a nonproliferation-specific protein; this S1 gene shares a high degree of homology to EF-1 alpha. We constructed specific riboprobes to the two genes, based on the difference in the 3' noncoding regions of both S1 and EF-1 alpha mRNAs. Northern analysis and RNase protection assays have revealed that S1 mRNA is present only in brain, heart, and muscle, while EF-1 alpha mRNA has been detected in all tissues surveyed so far. The same tissue specificity has been observed in mouse, suggesting that S1 expression is conserved between these two mammalian species. S1 transcript was detected in late brain embryogenesis (day 20), but in lower amounts than in 3-month-old adult brain. We show that the relative levels of both S1 and EF-1 alpha transcripts and their respective tissue abundances remain unchanged during the aging process. The function of S1 is not yet known; but these results suggest that it may be involved in specific control mechanisms for protein synthesis in tissues where cells (i.e. neurons and myocytes) are permanently locked in a state of nonproliferation.