Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla
Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla
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DOI：
10.1007/s11738-012-1199-0
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发表时间：
2013-01
影响因子：
2.6
通讯作者：
S. Yin;Wenyuan Gao;Yuyong Liang;Juan Wang;Hui Liu;Changlong Wei;Beimei Zuo
中科院分区：
文献类型：
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作者：
S. Yin;Wenyuan Gao;Yuyong Liang;Juan Wang;Hui Liu;Changlong Wei;Beimei Zuo
研究了太子参不定根的诱导以及蔗糖浓度和磷源对太子参生物量增长和代谢产物积累的影响。这些根最初在Murashige和Skoog培养基中培养4周。IBA 3.0 mg L-1是诱导不定根的最佳生长素，根的不定根诱导率（100%）高于叶片（78%）和茎（27%）。在蔗糖浓度为4%的培养基中，生物量最高，为1.04g/瓶干重，皂苷和多糖含量最高，分别为0.676%和24.4%。对于磷源，1.25 mM的磷酸盐浓度更有利于根的生物量（0.87 g/瓶DW），而最佳的皂苷（0.74%）和多糖（22.09%）达到2.5 mM的磷酸盐浓度。然而，2.5 mM磷酸盐下的皂苷含量与0.625 mM（0.69%）或3.75 mM磷酸盐（0.69%）下的皂苷含量没有显示出显著差异。
In this study, we investigated the induction ofPseudostellaria heterophyllaadventitious root and the effects of sucrose concentration and phosphate source on biomass increase and metabolites accumulation. These roots were initially cultured in Murashige and Skoog medium for 4 weeks. IBA 3.0 mg L−1proved to be the best auxin for inducing adventitious roots and the frequency of adventitious roots induced from roots (100 %) was higher than that from leaves (78 %) and stems (27 %). The medium with 4 % sucrose resulted in the optimum biomass i.e. 1.04 g/flask DW, and the content of saponin and polysaccharides reached the peak i.e. 0.676 and 24.4 %, respectively. With regards to phosphate source, 1.25 mM phosphate concentration was more favorable for biomass of roots (0.87 g/flask of DW), whereas the optimum saponin (0.74 %) and polysaccharides (22.09 %) were achieved with 2.5 mM phosphate. However, the saponin content at 2.5 mM phosphate did not show significant difference from the saponin content at 0.625 mM (0.69 %) or 3.75 mM phosphate (0.69 %).