Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent
Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent
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DOI：
10.1007/s00775-004-0560-1
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发表时间：
2004-09-01
影响因子：
3
通讯作者：
Bao, G
中科院分区：
文献类型：
--
作者：
Nitin, N;LaConte, LEW;Bao, G
我们报告了具有 PEG 修饰的磷脂胶束涂层的功能化超顺磁性氧化铁纳米颗粒的开发，以及将它们递送到活细胞中。通过动态光散射和电子显微镜测定，发现包覆颗粒的尺寸在12nm和14nm之间。 PEG-磷脂涂层具有高水溶性和稳定性，并且修饰的 PEG 的官能团允许各种部分的生物共轭，包括荧光染料和 Tat 肽。通过荧光显微镜、弛豫时间测量和磁共振成像（MRI）证实了功能化纳米粒子有效递送到活细胞中。这证明了使用尺寸均匀（类似于10 nm）的功能化磁性纳米粒子作为MRI造影剂用于深层组织细胞内分子成像的可行性。这些胶束包被的氧化铁纳米粒子提供了一个用于缀合各种部分的多功能平台，并且它们的小尺寸赋予了细胞内分子成像以最小的扰动的优势。
We report the development of functionalized superparamagnetic iron oxide nanoparticles with a PEG-modified, phospholipid micelle coating, and their delivery into living cells. The size of the coated particles, as determined by dynamic light scattering and electron microscopy, was found to be between 12 and 14 run. The PEG-phospholipid coating resulted in high water solubility and stability, and the functional groups of modified PEG allowed for bioconjugation of various moieties, including a fluorescent dye and the Tat peptide. Efficient delivery of the functionalized nanoparticles into living cells was confirmed by fluorescence microscopy, relaxation time measurements, and magnetic resonance imaging (MRI). This demonstrates the feasibility of using functionalized magnetic nanoparticles with uniform (similar to10 nm) sizes as an MRI contrast agent for intracellular molecular imaging in deep tissue. These micelle-coated iron oxide nanoparticles offer a versatile platform for conjugation of a variety of moieties, and their small size confers advantages for intracellular molecular imaging with minimal perturbation.