Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments
Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments
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DOI：
10.1042/bst0320209
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发表时间：
2004-04-01
影响因子：
3.9
通讯作者：
Banat, IM
中科院分区：
文献类型：
--
作者：
Rahman, TJ;Marchant, R;Banat, IM
在这项研究中，采用分子和基于培养的方法来表征与北爱尔兰地下土壤环境相关的嗜热细菌。从两个地点的地下土壤样品中分离出53种嗜热、好氧、产孢和不产孢的细菌。在16s rRNA基因测序之前，通过扩增核糖体DNA限制性内切分析筛选。大部分序列与热变形地杆菌（50%）和冷木犀地杆菌（34.6%）相关。分离株F10、F20和Tf与toebii Geobacillus F70的相似性仅为93%。因此，它们可能代表了土杆菌属的一个新种。
In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.