Microdialysis measurement of glucose in subcutaneous adipose tissue up to three weeks in Type 1 diabetic patients
Microdialysis measurement of glucose in subcutaneous adipose tissue up to three weeks in Type 1 diabetic patients
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DOI：
10.1016/s0300-2977(00)00022-x
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发表时间：
2000-07-01
影响因子：
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通讯作者：
Schoonen, AJM
中科院分区：
文献类型：
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作者：
Lutgers, HL;Hullegie, LM;Schoonen, AJM
背景资料：皮下脂肪组织的微透析可以提供连续监测葡萄糖的机会，当该装置连接到脑外葡萄糖传感器时。我们评估了我们的微透析探针是否能够在1型糖尿病患者中长时间测量脂肪组织葡萄糖。此外，腹部皮褶厚度和葡萄糖恢复之间的关系和自发性葡萄糖波动对其recovery.Methods的影响进行了评估：微透析探头成对插入皮下到腹部脂肪，并保持原位3周的8例1型糖尿病患者。在第1，3，4，8，11，16，和18天的探针保留，葡萄糖，通过微透析测量，比较毛细血管血糖浓度在4小时内。通过微透析获得的葡萄糖的回收率表示为毛细血管血糖concentration.Results的百分比：16个插入的探针（69%）中的11个是可评价的在整个研究。第1天和第3天的葡萄糖恢复率较低（分别为51 ± 23%和56 ± 18%，平均值± S.D.）与之后发现的值相比（第4、8、11、16和18天分别为67+/-19%，72+/-13%，76+/-14%，71+/-16%和76+/-18%，所有P < 0.05，与第1和3天相比）。皮褶厚度与3周血糖恢复呈负相关（r =-0.76; P < 0.03）。在很宽的范围内的毛细血管血糖concentration.Conclusions：延长在体内保留的微透析探针提高了恢复和降低1型糖尿病患者的脂肪组织采样葡萄糖的变异性。这些发现表明，基于微透析的葡萄糖测量提供了延长葡萄糖监测的机会。(C)2000爱思唯尔科技有限公司。保留所有权利。
Background: Microdialysis of subcutaneous adipose tissue may provide an opportunity to monitor glucose continuously, when the device is connected to an extracorporal glucose sensor. We assessed whether our microdialysis probes are capable of measuring adipose tissue glucose over a prolonged period in Type 1 diabetic patients. Furthermore, the relationship between abdominal skinfold thickness and glucose recovery and the effect of spontaneous glucose excursions on its recovery were evaluated.Methods: Microdialysis probes were pairwise inserted subcutaneously into the abdominal fat and remained in situ for 3 weeks in eight Type 1 diabetic patients. At days 1, 3, 4, 8, 11, 16, and 18 of probe retention, glucose, as measured by microdialysis, was compared to capillary blood glucose concentrations during a 4 h period. The recovery of glucose obtained by microdialysis was expressed as a percentage of the capillary blood glucose concentration.Results: Eleven of the 16 inserted probes (69%) were evaluable during the complete study. Recovery of glucose was lower at day 1 and 3 (51+/-23% and 56+/-18%, respectively, mean+/-S.D.) compared to values found afterwards (67+/-19%, 72+/-13%, 76+/-14%, 71+/-16%, and 76+/-18%, for day 4, 8, 11, 16, and 18, respectively, for all P < 0.05 vs. day 1 and 3). Skinfold thickness was inversely related to the overall 3 week glucose recovery (r = - 0.76; P < 0.03). Recovery was similar over a wide range of capillary blood glucose concentrations.Conclusions: Prolonged in vivo retention of microdialysis probes improves the recovery and lowers the variability of adipose tissue-sampled glucose in Type 1 diabetic patients. These findings show that microdialysis-based glucose measurements offer an opportunity for prolonged glucose monitoring. (C) 2000 Elsevier Science B.V. All rights reserved.