CD36 is involved in oleic acid detection by the murine olfactory system.
CD36 is involved in oleic acid detection by the murine olfactory system.
复制标题
CD36参与了鼠嗅觉系统的油酸检测。
DOI：
10.3389/fncel.2015.00366
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发表时间：
2015
影响因子：
5.3
通讯作者：
Neuhaus EM
中科院分区：
文献类型：
--
作者：
Oberland S;Ackels T;Gaab S;Pelz T;Spehr J;Spehr M;Neuhaus EM
嗅觉信号影响各种物种的食物摄入。为了最大限度地找到热量来源的机会，动物对脂肪食物和甘油三酯的偏好在嗅觉食物搜索行为中已经变得明显。然而，到目前为止，介导嗅觉依赖性脂肪酸识别的受体和配体的分子身份尚未描述。在这里，我们描述了一个子集的嗅觉感觉神经元表达的脂肪酸受体CD36和展示受体样定位的CD36在嗅觉纤毛STED显微镜。CD36阳性嗅神经元共享嗅觉特异性转导元件，并投射到腹侧嗅球中的许多肾小球。根据所描述的CD 36在其他感觉系统中作为脂肪酸受体或辅助受体的作用，在Ca 2+成像实验中，对牛奶主要成分油酸反应的嗅觉神经元数量在年轻的CD 36敲除小鼠中急剧减少。引人注目的是，我们还观察到显着的年龄依赖性的变化，CD36的定位，这是显着存在于睫状室只有在哺乳期。我们的研究结果支持CD36参与哺乳动物嗅觉系统的脂肪酸检测。
Olfactory signals influence food intake in a variety of species. To maximize the chances of finding a source of calories, an animal’s preference for fatty foods and triglycerides already becomes apparent during olfactory food search behavior. However, the molecular identity of both receptors and ligands mediating olfactory-dependent fatty acid recognition are, so far, undescribed. We here describe that a subset of olfactory sensory neurons expresses the fatty acid receptor CD36 and demonstrate a receptor-like localization of CD36 in olfactory cilia by STED microscopy. CD36-positive olfactory neurons share olfaction-specific transduction elements and project to numerous glomeruli in the ventral olfactory bulb. In accordance with the described roles of CD36 as fatty acid receptor or co-receptor in other sensory systems, the number of olfactory neurons responding to oleic acid, a major milk component, in Ca2+ imaging experiments is drastically reduced in young CD36 knock-out mice. Strikingly, we also observe marked age-dependent changes in CD36 localization, which is prominently present in the ciliary compartment only during the suckling period. Our results support the involvement of CD36 in fatty acid detection by the mammalian olfactory system.