SINDBIS VIRUS MESSENGER-RNA - 5'-TERMINI AND METHYLATED RESIDUES OF 2S-S-RNA AND 42-S-RNA
SINDBIS VIRUS MESSENGER-RNA - 5'-TERMINI AND METHYLATED RESIDUES OF 2S-S-RNA AND 42-S-RNA
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DOI：
10.1016/0042-6822(77)90471-8
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发表时间：
1977-01-01
期刊：
影响因子：
3.7
通讯作者：
GUILD, GM
中科院分区：
文献类型：
--
作者：
DUBIN, DT;STOLLAR, V;GUILD, GM
研究了Sindbis病毒感染的脊椎动物细胞[幼鼠肾脏BHK-21和鸡胚胎成纤维细胞]中积累的主要核糖核酸26种S和42种S的甲基化状态及其5‘末端的甲基化状态。将这些胞内物种与病毒42 S核糖核酸进行比较。所检测的5‘-末端都是封端的，结构为Mg(5’‘)pppAp。甲基化的鸟苷(Mg)帽由m22.7G和m32.2.7G组成，在细胞内RNA的情况下，m7G与病毒RNA不同，m7G只含有m7G。在细胞内RNA物种中掺入的甲基标记中，1/4-1/2以内部M5C残基的形式出现；病毒粒子RNA中没有这种残基。除了与病毒RNA不同外，Sindbis mRNAs还不同于宿主细胞的mRNAs，也不同于大多数其他脊椎动物细胞病毒所指定的mRNAs，其5‘末端的结构和内部甲基化残基的性质不同。文中还讨论了这些差异的可能影响。
The methylation status in general and the 5''-termini in particular of the main mRNA species, 26 S and 42 S RNA, that accumulate in Sindbis virus-infected vertebrate cells [baby hamster kidney BHK-21 and chicken embryo fibroblast] were studied. These intracellular species were compared with virion 42 S RNA. The 5''-termini examined were all capped and of the structure mG(5'')pppAp. The methylated guanosine (mG) caps consisted of m22.7G and m32.2.7G and m7G in the case of the intracellular RNAs, unlike virion RNA, which contained only m7G. Of the methyl label incorporated into the intracellular RNA species, 1/4-1/2 occurred as internal m5C residues; such residues were absent from virion RNA. In addition to differing from virion RNA, the Sindbis mRNAs differ from host cell mRNA and from the mRNA specified by most other viruses of vertebrate cells in the structure of their 5''-termini and in the nature of their internal methylated residues. Possible implications of these differences are discussed.