Comparative expression of p2x receptors and ecto-nucleoside triphosphate diphosphohydrolase 3 in hypocretin and sensory neurons in zebrafish
Comparative expression of p2x receptors and ecto-nucleoside triphosphate diphosphohydrolase 3 in hypocretin and sensory neurons in zebrafish
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DOI：
10.1016/j.brainres.2007.06.103
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发表时间：
2007-10-12
期刊：
影响因子：
2.9
通讯作者：
Mignot, Emmanuel
中科院分区：
文献类型：
--
作者：
Appelbaum, Lior;Skariah, Gemini;Mignot, Emmanuel
下丘脑分泌素/食欲素 (HCRT/ORX) 兴奋性神经肽在哺乳动物和鱼类的少量外侧下丘脑细胞中表达。在人类中，这些细胞的丧失会导致睡眠障碍发作性睡病。鉴定 HCRT 产生细胞中表达的基因可能会揭示睡眠的调节和发作性睡病的病理生理学。在这项研究中，进行了原位杂交分析来表征受体和酶的表达模式，这些受体和酶调节斑马鱼幼虫下丘脑分泌素细胞中 ATP 介导的传输。分离了编码外核苷三磷酸二磷酸水解酶 3 (ENTPD3/NTPDase3) 的斑马鱼 cDNA。正如之前在哺乳动物中报道的那样，该转录本被发现在斑马鱼 HCRT 细胞中表达。它还在脑神经（gV、gVII、gIV 和 gX）和脊髓中的初级感觉神经元（即 Rohon-Beard 神经元）中表达。接下来研究了已知斑马鱼 p2rx 嘌呤能受体家族成员的表达，发现与 entpd3 表达模式重叠。具体来说，p2rx2、p2rx3.1、p2rx3.2 和 p2rx8 在三叉神经节和 Rohon-Beard 神经元亚群中表达。与哺乳动物相反，p2rx2 在 HCRT 细胞中不表达；相反，p2rx8 在该下丘脑区域与 entpd3 一起表达。这些基因在脊椎动物的 HCRT 细胞和感觉神经元中表达的保守性表明 ATP 介导的传输在睡眠调节和感觉输入处理中发挥着重要作用。 (c) 2007 Elsevier B.V. 保留所有权利。
The hypocretin/orexin (HCRT/ORX) excitatory neuropeptides are expressed in a small population of lateral hypothalamic cells in mammals and fish. In humans, loss of these cells causes the sleep disorder narcolepsy. Identification of genes expressed in HCRT-producing cells may be revealing as to the regulation of sleep and the pathophysiology of narcolepsy. In this study, in situ hybridization analyses were performed to characterize the expression pattern of receptors and enzyme, which regulate ATP-mediated transmission in hypocretin cells of zebrafish larvae. The zebrafish cDNA encoding the ecto-nucleoside triphosphate diphosphohydrolase 3 (ENTPD3/NTPDase3) was isolated. This transcript was found to be expressed in zebrafish HCRT cells as previously reported in mammals. It was also expressed in the cranial nerves (gV, gVII, gIV and gX) and in primary sensory neurons (i.e., Rohon-Beard neurons) in the spinal cord. The expression of known zebrafish p2rx purinergic receptor family members was next studied and found to overlap with the entpd3 expression pattern. Specifically, p2rx2, p2rx3.1, p2rx3.2 and p2rx8 were expressed in the trigeminal ganglia and subsets of Rohon-Beard neurons. in contrast to mammals, p2rx2 was not expressed in HCRT cells; rather, p2rx8 was expressed with entpd3 in this hypothalamic region. The conservation of expression of these genes in HCRT cells and sensory neurons across vertebrates suggests an important role for ATP mediated transmission in the regulation of sleep and the processing of sensory inputs. (c) 2007 Elsevier B.V. All rights reserved.