Acid phosphatases in human plasma.
Acid phosphatases in human plasma.
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人血浆中的酸性磷酸酶。
DOI：
10.5555/uri:pii:002221437390108x
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
L. Yam
中科院分区：
文献类型：
--
作者：
chin;Robert A. Chuda;W. Lam;L. Yam
摘要采用生化方法测定人血浆和血清酸性磷酸酶的总酶活性，用聚丙烯酰胺凝胶电泳法测定同工酶谱。目的是确定血浆中各种同工酶的组织或细胞来源。正常血浆中含有抗酒石酸同工酶5，但红细胞或前列腺中没有可证实的同工酶。正常血清含有一种额外的同工酶3，它对酒石酸盐敏感，在凝血过程中从血小板中释放出来。在多种疾病中，血浆中可能出现其他同工酶。例如，在转移性前列腺癌中，血浆酶活性的变化是由于同工酶2的存在。许多酸性磷酸酶同工酶是细胞特异性的，即源自特定类型的细胞。血浆酸性磷酸酶的异常酶活性和酶谱模式经常反映疾病状态下组织的酶活性和酶谱模式。在一些血浆酸性磷酸酶同工酶异常的病例中，血浆总酶活性正常。本研究结果表明，凝胶电泳检测同工酶在追踪血浆中酶变化的原因方面比生化方法更敏感。
Abstract Human plasma and serum acid phosphatases are studied by biochemical methods to evaluate the total enzyme activity and by polyacrylamide gel electrophoresis to demonstrate the isoenzyme patterns. The aim is to identify the tissue or cell origin of the various isoenzymes in plasma. Normal plasma contains a tartrate-resistant isoenzyme 5 but no demonstrable isoenzymes from red blood cells or prostate. Normal serum contains an additional isoenzyme 3 which is tartrate-sensitive and is released from platelets during blood clotting. In a variety of diseases, other isoenzymes may appear in plasma. In metastatic prostate cancer, for example, the changes of plasma enzyme activity are due to the presence of isoenzyme 2. Many acid phosphatase isoenzymes are cell-specific, i.e., derived from a specific type of cell. Abnormal enzyme activity and zymogram patterns of plasma acid phosphatase frequently reflect those of tissues in disease conditions. In some of the cases with abnormal plasma acid phosphatase isoenzymes, the total enzyme activity in plasma is normal. Results of this study suggest that examination of the isoenzymes by gel electrophoresis is more sensitive than the biochemical methods in tracing the cause responsible for the enzyme changes in plasma.