Morphology of motoneurons in different subdivisions of the rat facial nucleus stained intracellularly with horseradish peroxidase
Morphology of motoneurons in different subdivisions of the rat facial nucleus stained intracellularly with horseradish peroxidase
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辣根过氧化物酶胞内染色大鼠面核不同分区运动神经元形态
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发表时间：
1986
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通讯作者：
E. Friauf
中科院分区：
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作者：
E. Friauf
将辣根过氧化物酶注射到大鼠面神经元内。通过逆行刺激颊神经、下颌缘神经或耳后神经分支来识别神经元。供应颊支的运动神经元胞体位于面神经核的外侧亚区，供应下颌缘支的运动神经元胞体位于面神经核的中间亚区，供应耳后支的运动神经元胞体位于面核的内侧亚区。用计算机辅助技术重建了11个运动神经元。它们的胞体直径平均为20μm，初生树突数平均为7.9个，树突树的总长度平均为17,650μm，3个运动神经元组间无明显差异。然而，根据重建的树突方向(即树突分布)，观察到三组运动神经元之间的主要差异。所有组的树突都从面核的边界延伸到网状结构中。中间分支和侧分支之间的边界被一些树枝晶越过，但重叠较小。相反，内侧亚区的运动神经元没有树突进入中间亚区，反之亦然。树突的范围完全由这两个亚区之间的边界所限制。然而，在Nissl定义的核边界之外，来自相邻分区中的细胞的树突重叠。
Horseradish peroxidase was injected into single facial motoneurons of the rat. Neurons were identified by antidromic stimulation of either the buccal or the marginal mandibular or the posterior auricular nerve branches. Motoneuronal cell bodies supplying the buccal branch were located in the lateral subdivision of the facial nucleus, those supplying the marginal mandibular branch were in the intermediate subdivision, and those supplying the posterior auricular branch were in the medial subdivision. Eleven motoneurons were reconstructed with a computer‐assisted technique. Their soma diameters averaged 20 μm; the average number of primary dendrites was 7.9 and the combined lengths of the dendritic trees averaged 17,650 μm. There was no distinction between the three motoneuron groups in terms of these and other quantitative data. However, on the basis of reconstructed dendritic tree orientation (i.e., dendritic distribution), major differences were observed between motoneurons of the three groups. Dendrites from all groups extended beyond the boundaries of the facial nucleus into the reticular formation. The border between the intermediate and the lateral subdivision was crossed by some dendrites but the overlap was small. In contrast, no dendrite of a motoneuron in the medial subdivision entered the intermediate subdivision and vice versa. The dendritic extent was totally restricted by the borders between these two subdivisions. Outside the Nissl‐defined nuclear border, however, dendrites from cells in adjacent subdivisions overlapped.