CYCLIC ADP-RIBOSE REGULATION OF RYANODINE RECEPTORS INVOLVED IN AGONIST-EVOKED CYTOSOLIC CA2+ OSCILLATIONS IN PANCREATIC ACINAR-CELLS
CYCLIC ADP-RIBOSE REGULATION OF RYANODINE RECEPTORS INVOLVED IN AGONIST-EVOKED CYTOSOLIC CA2+ OSCILLATIONS IN PANCREATIC ACINAR-CELLS
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DOI：
10.1002/j.1460-2075.1994.tb06478.x
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发表时间：
1994-05-01
期刊：
影响因子：
11.4
通讯作者：
PETERSEN, OH
中科院分区：
文献类型：
--
作者：
THORN, P;GERASIMENKO, O;PETERSEN, OH
我们研究了ryanodine敏感的细胞内Ca ~（2+）释放通道（ryanodine受体）在乙酰胆碱（ACh）或胆囊收缩素（CCK）引起的胰腺腺泡细胞胞浆Ca ~（2+）振荡中的作用。Ryanodine可消除或显著抑制激动剂诱发的Ca ~（2+）峰化，但可增强直接应用三磷酸肌醇（InsP（3））诱发的峰化频率。我们还研究了可能性，环ADP-核糖（cADP-核糖），推定的第二信使控制的兰尼碱受体，在Ca 2+振荡中发挥作用。我们发现cADP-核糖本身可诱导位于分泌极的重复性Ca ~（2+）峰，这些峰可被ryanodine阻断，但也可被InsP（3）受体拮抗剂肝素阻断。我们的结果表明，ryanodine和InsP（3）受体都参与了Ca 2+峰的产生。
We have investigated the role of the ryanodine-sensitive intracellular Ca2+ release channel (ryanodine receptor) in the cytosolic Ca2+ oscillations evoked in pancreatic acinar cells by acetylcholine (ACh) or cholecystokinin (CCK). Ryanodine abolished or markedly inhibited the agonist evoked Ca2+ spiking, but enhanced the frequency of spikes evoked by direct internal inositol trisphosphate (InsP(3)) application. We have also investigated the possibility that cyclic ADP-ribose (cADP-ribose), the putative second messenger controlling the ryanodine receptor, plays a role in Ca2+ oscillations. We found that cADP-ribose could itself induce repetitive Ca2+ spikes localized in the secretory pole and that these spikes were blocked by ryanodine, but also by the InsP(3) receptor antagonist heparin. Our results indicate that both the ryanodine and the InsP(3) receptors are involved in Ca2+ spike generation.