GENES FOR BREAKFAST - THE HAVE-YOUR-CAKE-AND-EAT-IT-TOO OF BACTERIAL TRANSFORMATION
GENES FOR BREAKFAST - THE HAVE-YOUR-CAKE-AND-EAT-IT-TOO OF BACTERIAL TRANSFORMATION
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DOI：
10.1093/oxfordjournals.jhered.a111361
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发表时间：
1993-09-01
影响因子：
3.1
通讯作者：
REDFIELD, RJ
中科院分区：
文献类型：
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作者：
REDFIELD, RJ
细菌转化，其中细胞吸收并重组游离的DNA链，是被认为进化为遗传交换的最简单过程，即，因为产生具有重组基因型的后代的潜在益处。然而，其他两个功能也同样合理：获得完整的DNA链用于DNA损伤的重组修复，以及获得DNA分子中所含的营养物质。虽然通过转化产生的重组后代可能是有益的，但导致转化的基因的成功受到其他因素的限制，特别是受到来自死细胞的DNA的遗传质量的限制。我们最近在自然转化细菌流感嗜血杆菌和枯草芽孢杆菌中的实验表明，DNA修复假说不太可能是正确的。 In H.在流感病毒中，转化并没有可检测地增加细胞在DNA损伤中存活的能力。更重要的是，我们发现，尽管营养限制诱导了H.流感病毒和B.枯草芽孢杆菌中，它不是由DNA损伤引起的。因此，我们倾向于这样的假设，即转化是作为一个营养吸收系统进化的，特别是因为在许多自然转化细菌的环境中，不相关的DNA是丰富的。
Bacterial transformation, in which cells take up and recombine free strands of DNA, is the simplest process thought to have evolved for genetic exchange, i.e., because of the potential benefits of producing progeny with recombinant genotypes. However, two other functions are equally plausible: acquisition of intact DNA strands to use for recombinational repair of DNA damage, and acquisition of the nutrients contained in DNA molecules. Although the recombinant progeny produced by transformation can be beneficial, the success of genes causing transformation is limited by other factors, especially by the genetic quality of DNA derived from dead cells. Our recent experiments in the naturally transformable bacteria Haemophilus influenzae and Bacillus subtilis suggest that the DNA-repair hypothesis is unlikely to be correct. In H. influenzae, transformation does not detectably increase the cells' ability to survive DNA damage. More importantly, we have found that, although competence (the ability to take up DNA) is induced by nutritional limitation in both H. influenzae and B. subtilis, it is not induced by DNA damage in either. Thus we favor the hypothesis that transformation evolved as a nutrient-uptake system, especially because unrelated DNA is abundant in the environments of many naturally transformable bacteria.