Carbon nanospheres for fluorescent biomolecular detection
Carbon nanospheres for fluorescent biomolecular detection
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DOI：
10.1039/c0jm04107f
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Sun, Xuping
中科院分区：
文献类型：
--
作者：
Li, Hailong;Zhang, Yingwei;Sun, Xuping
在这篇文章中，我们展示了碳纳米球的大规模的简易制备，通过处理石墨与氯磺酸和它们的使用作为非常有效的淬灭剂，用于高灵敏度和选择性的核酸和凝血酶检测。该检测通过两个步骤完成：（1）碳纳米球（CNS）吸附并猝灭染料标记的单链DNA（ssDNA）探针的荧光;（2）在靶标存在下，生物分子相互作用抑制荧光猝灭，这使得ssDNA从CNS表面脱离，导致染料荧光恢复。
In this article, we demonstrate the facile preparation of carbon nanospheres on a large scale by treating graphite with chlorosulfonic acid and their use as very effective quenchers for highly sensitive and selective nucleic acid and thrombin detection. The detection is accomplished by two steps: (1) a carbon nanosphere (CNS) adsorbs and quenches the fluorescence of a dye-labeled single-stranded DNA (ssDNA) probe; (2) in the presence of a target, the biomolecular mutual interaction suppresses fluorescence quenching, which makes the ssDNA detach from the CNS surface, leading to recovery of the dye fluorescence.