Toward Mail-in-Sensors for SARS-CoV-2 Detection: Interfacing Gel Switch Resonators with Cell-Free Toehold Switches
Toward Mail-in-Sensors for SARS-CoV-2 Detection: Interfacing Gel Switch Resonators with Cell-Free Toehold Switches
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DOI：
10.1021/acssensors.1c02450
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发表时间：
2022-03-25
期刊：
影响因子：
8.9
通讯作者：
Reuel, Nigel F.
中科院分区：
文献类型：
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作者：
Carr, Adam R.;Dopp, Jared L.;Reuel, Nigel F.
COVID-19疫情强调了预防性检测对控制传染病传播的重要性。自大流行开始以来，病毒和抗体检测方法的快速发展、扩大规模和部署大大提高了检测能力。检测方法的理想属性是低产品成本，自我管理的协议，并能够在密封的信封邮寄的安全分析和随后的记录到公共卫生数据库。在这里，这样的平台被证明与丝网印刷，电感器-电容器（LC）谐振器作为传感器和支点开关耦合与细胞表达作为生物选择性抑制元件。在存在来自SARS-CoV-2的N基因的情况下，支点开关松弛，蛋白酶表达，并且其降解明胶开关，该明胶开关最终改变平面谐振传感器的谐振频率。明胶开关谐振器（GSR）可以通过一个密封的信封进行分析，允许进行评估，而不需要小心的样品处理与个人防护设备或需要与其他试剂的后处理。该传感器中使用的立足点开关对SARS-CoV-2病毒的选择性超过了对3种季节性冠状病毒和SARS-CoV-1的选择性，检测限为100拷贝/μ L。该平台的功能和评估在一个密封的信封与自动扫描仪显示与隔夜装运，并讨论了进一步的改进，以增加信号的稳定性和进一步简化用户协议，以邮件在平台
The COVID-19 pandemic has emphasized the importance of widespreadtesting to control the spread of infectious diseases. The rapid development, scale-up, anddeployment of viral and antibody detection methods since the beginning of the pandemichave greatly increased testing capacity. Desirable attributes of detection methods are lowproduct costs, self-administered protocols, and the ability to be mailed in sealed envelopes forthe safe analysis and subsequent logging to public health databases. Herein, such a platform isdemonstrated with a screen-printed, inductor-capacitor (LC) resonator as a transducer anda toehold switch coupled with cell-free expression as the biological selective recognitionelement. In the presence of the N-gene from SARS-CoV-2, the toehold switch relaxes,protease enzyme is expressed, and it degrades a gelatin switch that ultimately shifts theresonant frequency of the planar resonant sensor. The gelatin switch resonator (GSR) can beanalyzed through a sealed envelope allowing for assessment without the need for carefulsample handling with personal protective equipment or the need for workup with otherreagents. The toehold switch used in this sensor demonstrated selectivity to SARS-CoV-2 virus over three seasonal coronavirusesand SARS-CoV-1, with a limit of detection of 100 copies/mu L. The functionality of the platform and assessment in a sealed envelopewith an automated scanner is shown with overnight shipment, and further improvements are discussed to increase signal stability andfurther simplify user protocols toward a mail-in platform