Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation
Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation
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DOI：
10.1007/s12565-019-00479-1
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发表时间：
2019-02
影响因子：
1.2
通讯作者：
M. To;C. Su;Kouki Hidaka;T. Okudera;M. Matsuo
中科院分区：
文献类型：
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作者：
M. To;C. Su;Kouki Hidaka;T. Okudera;M. Matsuo
已经开发了几种方法来再生丢失的牙槽骨。富含血小板的纤维蛋白（PRF）是一种有用的牙科新骨形成的辅助剂。为了阐明先进的PRF（A-PRF）对骨形成的影响，我们将A-PRF凝块插入拔牙后的牙槽窝。从比格犬中拔出前磨牙，并将A-PRF施加到牙槽中。采用组织学和免疫荧光检查评估新骨形成，并在术后14天和30天评估骨形成率。组织学检查显示，A-PRF组术后14 d有新生骨填充至骨槽中心，30 d后多孔骨内有粗大、规则的骨小梁排列。与对照组相比，A-PRF组新生骨中骨钙素和骨桥蛋白的表达更高。A-PRF组的骨形成率也高于对照组。因此，A-PRF应用可以导致增强的新骨形成，并且可以有助于加速骨形成。A-PRF通过增强成骨细胞的活性，在诱导骨形成过程中比自限性过程更快，可能在临床医学中用于骨形成。
Several methods have been developed to regenerate lost alveolar bone. Platelet-rich fibrin (PRF) is a useful adjunct for new bone formation in dentistry. To elucidate the effect of advanced PRF (A-PRF) on bone formation, we inserted A-PRF clots in sockets after tooth extraction. Premolars were extracted from beagle dogs, and A-PRF was applied to the socket. New bone formation was assessed using histological and immunofluorescence examinations, and the bone formation ratio was evaluated 14 and 30 days postoperatively. Histological examination revealed newly formed bone filling the sockets up to the center in the A-PRF group at 14 days postoperatively, while thick and regular bone trabeculae were arranged in porous bone after 30 days. Higher expressions of osteocalcin and osteopontin were observed in newly formed bone in the A-PRF group, compared to the control group. The bone formation ratio was also higher in the A-PRF group than in the control group. Thus, A-PRF application may result in enhanced new bone formation and may aid in accelerating bone formation. A-PRF was more rapid than a self-limiting process during induction of bone formation by enhancing osteoblast activity and may be useful for bone formation in clinical medicine.