Impact of fasting time on hepatic lipid metabolism in nutritional animal studies
Impact of fasting time on hepatic lipid metabolism in nutritional animal studies
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DOI：
10.1080/09168451.2014.923297
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发表时间：
2014-09-01
影响因子：
1.6
通讯作者：
Komai, Michio
中科院分区：
文献类型：
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作者：
Ikeda, Ikuo;Metoki, Kanae;Komai, Michio
许多关于改善脂质代谢的动物研究，使用饮食成分，在喂养的最后一天禁食动物。虽然禁食对脂质代谢有显著影响，但其时间依赖性影响尚未完全了解。我们研究了几个禁食时间对脂质代谢的影响。用半合成饲料喂养2周的大鼠在禁食0（9：00 am）、6（7：00 am-1：00 pm）、9（0：00 am-9：00 am）和13 h（8：00 pm-9：00 am）后处死。与0 h组相似，9 h和13 h组肝重和肝三酰甘油含量显著降低。参与脂肪酸合成的肝酶活性在禁食期间逐渐降低。相反，观察到酶的基因表达的急剧时间依赖性减少。肉碱棕榈酰转移酶mRNA的表达在禁食组高于0 h组。我们的研究表明，禁食对大鼠肝脏脂质代谢相关的几个参数有显着影响。
Many animal studies on improvement of lipid metabolism, using dietary components, fast the animals on the final day of the feeding. Although fasting has a significant impact on lipid metabolism, its time-dependent influence is not fully understood. We examined the effects of several fasting times on lipid metabolism. Rats fed with a semisynthetic diet for 2 wk were killed after 0 (9:00 am), 6 (7:00 am-1:00 pm), 9 (0:00 am-9:00 am), and 13 h (8:00 pm-9:00 am) of fasting. Co mpared to the 0 h group, marked reduction of liver weight and hepatic triacylglycerol content was observed in the 9 and 13 h groups. Activities of hepatic enzymes involved in fatty acid synthesis gradually decreased during fasting. In contrast, drastic time-dependent reduction of gene expression, of the enzymes, was observed. Expression of carnitine palmitoyltransferase mRNA was higher in the fasting groups than in the 0 h group. Our study showed that fasting has a significant impact on several parameters related to lipid metabolism in rat liver.