A Transcription Factor STOP1-Centered Pathway Coordinates Ammonium and Phosphate Acquisition in Arabidopsis.
A Transcription Factor STOP1-Centered Pathway Coordinates Ammonium and Phosphate Acquisition in Arabidopsis.
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DOI：
10.1016/j.molp.2021.06.024
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发表时间：
2021-06
期刊：
影响因子：
27.5
通讯作者：
W. Tian;J. Ye;M. Cui;Jun Bo Chang;Yu Liu;Gui-Xin Li;Yunrong Wu;J. Xu;N. Harberd;C. Mao
中科院分区：
文献类型：
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作者：
W. Tian;J. Ye;M. Cui;Jun Bo Chang;Yu Liu;Gui-Xin Li;Yunrong Wu;J. Xu;N. Harberd;C. Mao
磷是植物生长发育所必需的大量营养元素。天然磷酸盐（Pi）储量是有限的，因此更好地了解作物对Pi的利用对全球粮食安全至关重要。长期以来，铵被认为可以提高农业中磷的获得效率;然而，协调磷营养和铵的分子机制仍然不清楚。在这里，我们发现，铵是一种新的引发剂，刺激积累的一个关键的调节蛋白，STOP 1，在细胞核ofopsisroot细胞下Pi缺乏。我们表明，磷缺乏促进铵吸收介导的AMT 1转运蛋白，并导致快速酸化的根表面。微球酸化触发的STOP 1积累激活有机酸的排泄，这有助于从不溶性铁或钙磷酸盐中溶解Pi。AMT 1转运蛋白对铵的摄取受CIPK 23蛋白激酶的下调，当铵达到毒性水平时，CIPK 23蛋白激酶的表达受STOP 1的直接调节。综上所述，我们已经确定了一个以STOP 1为中心的调控网络，该网络将外部铵与来自不溶性磷酸盐来源的有效Pi获取联系起来。这些研究结果提供了一个框架，制定可能的战略，以提高作物产量，通过提高利用土壤中的非生物可利用的养分。
Phosphorus (P) is an indispensable macronutrient required for plant growth and development. Natural phosphate (Pi) reserves are finite, and a better understanding of Pi utilization by crops is therefore vital for worldwide food security. Ammonium has long been known to enhance Pi acquisition efficiency in agriculture; however, the molecular mechanisms coordinating Pi nutrition and ammonium remains unclear. Here, we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein, STOP1, in the nuclei ofArabidopsisroot cells under Pi deficiency. We show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root surface. Rhizosphere acidification-triggered STOP1 accumulation activates the excretion of organic acids, which help to solubilize Pi from insoluble iron or calcium phosphates. Ammonium uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic levels. Taken together, we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate sources. These findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil.