Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism
Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism
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DOI：
10.1136/ard.2004.022590
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发表时间：
2005-02-01
影响因子：
27.4
通讯作者：
Lapadula, G
中科院分区：
文献类型：
--
作者：
Iannone, F;De Bari, C;Lapadula, G
目的：为了确定Bcl-2，p53，Fas/CD 95是否有助于控制软骨metabolis.Methods：6个正常和14个骨关节炎（OA）软骨样本进行了检查，并从每个样本显示最小（Min）和最大（Max）解剖损伤的两个区域被选择。通过连续酶消化和新鲜处理分离软骨细胞。免疫荧光和流式细胞仪分析Bcl-2、p53和Fas/CD 95的表达;碘化丙啶分析细胞周期;[H-3]胸苷掺入法检测软骨细胞增殖。结果：Max组细胞内Bcl-2表达水平（27.5%）显著高于Min组（21%，P
Objective: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism.Methods: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [H-3]thymidine incorporation.Results: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p