Anticipating endoplasmic reticulum stress: A novel early response before pathogenesis-related gene induction
Anticipating endoplasmic reticulum stress: A novel early response before pathogenesis-related gene induction
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DOI：
10.2307/3871088
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发表时间：
1999-10-01
期刊：
影响因子：
11.6
通讯作者：
Denecke, J
中科院分区：
文献类型：
--
作者：
Jelitto-Van Dooren, EPWM;Vidal, S;Denecke, J
当受到病原体攻击时，植物会产生一系列防御相关蛋白。其中许多由粗面内质网（RER）合成，从细胞中分泌或沉积在液泡中。编码内质网（ER）驻留分子伴侣的基因，如内腔结合蛋白（BiP），也在这些条件下诱导。在这里，我们表明，BiP诱导发生系统性的整个工厂。此外，这种诱导发生迅速，并先于编码病程相关（PR）蛋白的基因的表达。潜在的信号转导途径被证明是独立的信号分子水杨酸和未折叠的蛋白质反应途径。此外，BiP诱导不依赖于PR基因的诱导。单独的BiP过量生产不足以引起PR基因表达的诱导;然而，通过叠加ER应激限制ER腔中BiP的量抑制PR基因表达的诱导。我们建议，诱导BiP的表达在植物-病原体相互作用是必需的，作为早期反应，以支持PR蛋白合成的RER和一种新的信号转导途径存在触发这种快速反应。
When it is attacked by a pathogen, a plant produces a range of defense-related proteins. Many of these are synthesized by the rough endoplasmic reticulum (RER) to be secreted from the cell or deposited in vacuoles. Genes encoding endoplasmic reticulum (ER)-resident chaperones, such as the lumenal binding protein (BiP), are also induced under these conditions. Here, we show that BiP induction occurs systemically throughout the plant. Furthermore, this induction occurs rapidly and precedes expression of genes encoding pathogenesis-related (PR) proteins. The underlying signal transduction pathway was shown to be independent of the signaling molecule salicylic acid and the unfolded protein response pathway. In addition, BiP induction was independent of PR gene induction. Overproduction of BiP alone was not sufficient to cause induction of PR gene expression; however, limiting the amount of BiP in the ER lumen via superimposed ER stress inhibited the induction of PR gene expression. We propose that the induction of BiP expression during plant-pathogen interactions is required as an early response to support PR protein synthesis on the RER and that a novel signal transduction pathway exists to trigger this rapid response.