Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
复制标题
短刺海胆中间型海胆消化器官纤维素酶的纯化和生化表征
DOI：
10.1007/s12562-012-0528-y
复制
发表时间：
2012
影响因子：
1.9
通讯作者：
Y. Takagi
中科院分区：
文献类型：
--
作者：
Syuto Hasegawa;K. Ura;Hiroyuki Tanaka;T. Ojima;Y. Takagi
我们从短刺海胆Strogylocentrotus intermedius的消化器官中分离出一种纤维素酶，该纤维素酶使用离子交换层析和凝胶过滤的组合，并测定羧甲基纤维素酶的活性。分离的纤维素酶经刚果红染色呈单一条带。在还原条件下通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的分离的纤维素酶的分子量为59 kDa。该纤维素酶对羧甲基纤维素具有水解活性，最适温度为30 °C，最适pH为8.0。酶的热稳定性通过测定在pH 7.0下处理30分钟时活性降低50%的温度来表征，发现为32 °C。纤维素酶活性在5-20 °C保持在高水平，这是短刺海胆的生长温度。这些结果证实，短刺海胆最好在<20 °C的水温下饲养。
We isolated a cellulase from the digestive organs of the short-spined sea urchin Strogylocentrotus intermedius using a combination of ion-exchange chromatography and gel filtration together with an assay for carboxymethylcellulase activity. The isolated cellulase was stained as a single band by Congo red. The molecular weight of the isolated cellulase, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, was 59 kDa. The isolated cellulase exhibited hydrolytic activity toward carboxymethyl cellulose, with an optimum temperature and pH of 30 °C and pH 8.0, respectively. The thermal stability of the enzyme was characterized by determining the temperature at which activity decreased by 50 % with treatment for 30 min at pH 7.0 and found to be 32 °C. Cellulase activity remained at a high level at 5–20 °C, which is the growth temperature of the short-spined sea urchin. These results confirm that the short-spined sea urchin should preferably be reared at a water temperature of <20 °C.
海胆胚胎原代间充质细胞表达的 mRNA 的大规模分析。
DOI：
10.1242/dev.128.13.2615
发表时间：
2001
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Zhu,X;Mahairas,G;Illies,M;Cameron,RA;Davidson,EH;Ettensohn,CA
通讯作者：
Ettensohn,CA