AN ANALYSIS OF THE COMPLETE SEQUENCE OF A SUGARCANE BACILLIFORM VIRUS GENOME INFECTIOUS TO BANANA AND RICE
AN ANALYSIS OF THE COMPLETE SEQUENCE OF A SUGARCANE BACILLIFORM VIRUS GENOME INFECTIOUS TO BANANA AND RICE
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DOI：
10.1099/0022-1317-74-1-15
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发表时间：
1993-01-01
影响因子：
3.8
通讯作者：
OLSZEWSKI, NE
中科院分区：
文献类型：
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作者：
BOUHIDA, M;LOCKHART, BEL;OLSZEWSKI, NE
甘蔗杆状病毒(ScBV)是一种棒状病毒，其基因组由环状dsDNA组成。本文报道了一个已克隆的感染性ScBV基因组的全序列。基因组大小为7568bp，具有一系列特征，表明ScBV是一种副病毒。存在一个tRNA(Met)结合位点，可作为负链合成的引物。ScBV基因组的正链包含三个开放阅读框架(ORF)，它们能够编码M(R)值分别为22K、13K和215K的蛋白质。215K蛋白具有与RNA结合域、天冬氨酸蛋白酶和逆转录酶复制酶相似的区域。此外，215K蛋白还有一个区域与植物病毒的胞间运输蛋白具有有限的相似性。与其他已测序的水稻黄斑驳病毒(CoYMV)和水稻通草杆状病毒(RTBV)的比较表明，这些病毒中ORF的排列是保守的。位于假定的RNA结合域旁边的是Badna病毒独有的富含半胱氨酸的区域。当确定部分植物副病毒逆转录酶的分子关系时，CoYMV和ScBV形成一个不同的簇，而花椰菜花叶病毒、康乃馨蚀环病毒和罗汉果花叶病毒形成第二个簇。巴德纳病毒RTBV和菜杆病毒大豆褪绿斑驳病毒占据了簇之间的中间位置。当通过农杆菌介导法接种时，含有1.1个拷贝的克隆ScBV基因组的构建体对水稻和香蕉都具有侵染性。
The genome of sugarcane bacilliform virus (ScBV), a badnavirus, consists of a circular dsDNA. The complete sequence of a cloned infective ScBV genome is reported here. The genome is 7568 bp in size and possesses a number of features suggesting that ScBV is a pararetrovirus. A tRNA(Met)- binding site that may serve as a primer for minus-strand synthesis is present. The plus-strand of the ScBV genome contains three open reading frames (ORFs) which are capable of encoding proteins with calculated M(r) values of 22K, 13K and 215K. The 215K protein has regions with similarity to the RNA-binding domains, aspartic proteases and replicases of retro-elements. In addition, the 215K protein also has a region with restricted similarity to the intercellular transport proteins of plant viruses. Comparisons with the other sequenced badnaviruses, Commelina yellow mottle (CoYMV) and rice tungro bacilliform (RTBV) viruses, indicate that the arrangement of the ORFs in these viruses is conserved. Located next to the putative RNA-binding domain is a cysteine-rich region that is unique to the badnaviruses. When the molecular relationships of a portion of the reverse transcriptases of plant pararetroviruses were determined, two badnaviruses, CoYMV and ScBV, form one distinct cluster, whereas three caulimoviruses, cauliflower mosaic virus, carnation etched ring virus and figwort mosaic virus, form a second cluster. The badnavirus RTBV and the caulimovirus soybean chlorotic mottle virus occupy intermediate positions between the clusters. When introduced by Agrobacterium-mediated inoculation, a construct containing 1.1 copies of the cloned ScBV genome is infectious to both rice and banana.