Lipid peroxidation and antielastase activity in the lung under oxidant stress: role of antioxidant defenses.
Lipid peroxidation and antielastase activity in the lung under oxidant stress: role of antioxidant defenses.
复制标题
氧化应激下肺中的脂质过氧化和抗弹性蛋白酶活性：抗氧化防御的作用。
DOI：
10.1152/jappl.1991.70.4.1456
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Mohsenin,V
中科院分区：
文献类型：
--
作者：
Mohsenin,V
在氧化应激下，研究了脂质过氧化在人类受试者肺泡衬里液中α1-蛋白酶抑制剂（α1-PI）失活中的作用。暴露于 4 ppm 二氧化氮 (NO2) 3 小时会导致肺泡内壁液中脂质过氧化产物的量显着增加，其中共轭二烯是主要种类。在接触 NO2 之前补充维生素 C 和 E 4 周可显着降低共轭二烯的水平（对照为 804 +/- 103 pmol/微克总磷脂，而补充维生素为 369 +/- 58，P = 0.003）。尽管在灌洗液中可检测到丙二醛，但其对脂质过氧化产物总量的贡献很小，并且两组中的水平相当。在不补充维生素的情况下暴露 NO2 会导致对照组肺泡内壁液的弹性蛋白酶抑制能力 (EIC) 显着下降，但补充维生素组则没有显着下降[对照 3.67 +/- 0.32 微克 α 1-PI/微克猪胰腺弹性蛋白酶 (PPE) 与补充维生素的 2.75 +/- 0.17，P 小于0.03]。补充维生素组的共轭二烯水平较低，EIC 较高。相反，对照组的灌洗液中具有较高水平的共轭二烯和较低的 EIC。这些研究表明，脂质过氧化是正常受试者肺部暴露于氧化剂期间的早期事件。灌洗液中脂质过氧化产物的出现与肺泡内壁液 EIC 的降低有关。维生素 C 和 E 可减少脂质过氧化，并在氧化应激期间保持下呼吸道液体的 EIC。
The role of lipid peroxidation in the inactivation of alpha 1-protease inhibitor (alpha 1-PI) in the alveolar lining fluid of human subjects has been examined under oxidant stress. Exposure to nitrogen dioxide (NO2) at 4 ppm for 3 h resulted in a significant increase in the amount of lipid peroxidation products in the alveolar lining fluid, with conjugated dienes the predominant species. Four-week supplementation with vitamins C and E before NO2 exposure markedly decreased the levels of conjugated dienes (control 804 +/- 103 pmol/micrograms total phospholipids vs. vitamin-supplemented 369 +/- 58, P = 0.003). Malondialdehydes, although detectable in the lavage fluid, contributed little to the total amount of lipid peroxidation products, and the levels were comparable in both groups. NO2 exposure in the absence of vitamin supplementation caused a significant decrease in the elastase inhibitory capacity (EIC) of the alveolar lining fluid in the control group but not in the vitamin-supplemented group [control 3.67 +/- 0.32 micrograms alpha 1-PI/micrograms porcine pancreatic elastase (PPE) vs. vitamin-supplemented 2.75 +/- 0.17, P less than 0.03]. The vitamin-supplemented group had a lower level of conjugated dienes and a higher EIC. Conversely, the control group had higher levels of conjugated dienes and a lower EIC in their lavage fluid. These studies demonstrate that lipid peroxidation occurs as an early event during oxidant exposure in the lungs of normal subjects. The appearance of lipid peroxidation products in the lavage fluid is associated with a decrease in the EIC of the alveolar lining fluid. Vitamins C and E diminish lipid peroxidation and preserve the EIC of the lower respiratory tract fluid during oxidant stress.