Effect of sonication parameters on transdermal delivery of insulin to hairless rats
Effect of sonication parameters on transdermal delivery of insulin to hairless rats
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DOI：
10.1016/s0168-3659(02)00054-8
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发表时间：
2002-05-17
影响因子：
10.8
通讯作者：
Patat, F
中科院分区：
文献类型：
--
作者：
Boucaud, A;Garrigue, MA;Patat, F
低频超声的应用已被证明可以促进胰岛素等大分子药物的经皮转运。在这项研究中，我们研究了超声诱导的胰岛素透皮给药对超声参数的依赖。胰岛素在无毛大鼠体内使用20千赫超声施加在超声强度，应用时间和脉冲长度范围内。监测动物血糖水平的变化，以评估胰岛素的转运。结果显示了一个阈值，低于这个阈值，每个超声参数都没有观察到血糖水平的可检测变化。此外，我们的研究结果表明，超声增强依赖于能量剂量和超声脉冲的长度，这与基于空化的机制一致。超声应用时间小于15 min的降糖效果更为显著，与皮下注射0.5 U胰岛素效果相似。超声预处理无毛大鼠皮肤后应用胰岛素，血糖水平无明显改变，说明胰岛素的透皮转运主要发生在超声过程中。因此，在胰岛素依赖型糖尿病的治疗中，声泳术可能被应用于无创无痛的胰岛素输送。(C) 2002 Elsevier Science B.V.版权所有
Application of low-frequency ultrasound has been shown to enhance transdermal drug transport of large molecules such as insulin. In this study, we investigated the dependence of ultrasound-induced transdermal delivery of insulin on ultrasound parameters. Insulin was delivered in vivo to hairless rats using 20 kHz ultrasound applied over a range of ultrasound intensity, application time and pulse length. Change in blood glucose levels of the animals was monitored to assess insulin transport. The results showed a threshold below which no detectable changes in blood glucose level was observed for each ultrasound parameter. Moreover, our findings indicated that sonophoretic enhancement is dependent on energy dose and length of ultrasound pulse that is consistent with a cavitation-based mechanism.. The more significant effect of lowering glycemia was obtained with application of less than 15 min ultrasound and was similar to subcutaneous injection of 0.5 U of insulin. Pretreatment of hairless rat skin with ultrasound followed by application of insulin resulted in no significant modification in blood glucose level, indicating that transdermal transport of insulin mainly occurred during sonication. Sonophoresis may therefore potentially be applied for non-invasive and painless delivery of insulin in the treatment of insulin-dependent diabetes. (C) 2002 Elsevier Science B.V. All rights reserved.