Trehalose is an important mediator of cap1p oxidative stress response in Candida albicans
Trehalose is an important mediator of cap1p oxidative stress response in Candida albicans
复制标题
海藻糖是白色念珠菌 Cap1p 氧化应激反应的重要介质。
DOI：
10.1248/bpb.31.421
复制
发表时间：
2008-03-01
影响因子：
2
通讯作者：
Zhang, GuoQing
中科院分区：
文献类型：
--
作者：
Cao, YingYing;Wang, Yan;Zhang, GuoQing
海藻糖是一种非还原性二糖，在稳定期或氧化应激下大量积累，是多种生物体的应激保护剂。在这里，我们研究了海藻糖与Cap 1 p的假定相关性，Cap 1 p是一种碱性亮氨酸拉链（bZip）转录因子，参与白念珠菌的氧化应激耐受。HPLC-MS分析表明，海藻糖没有积累在cap 1/cap 1突变体在稳定期。当突变体暴露于高浓度的H2 O2时，海藻糖的积累仍然没有被诱导。在上述两种条件下，cap 1/cap 1突变体对H2 O2均表现出较高的敏感性，但细胞活力较低。此外，当向cap 1/cap 1突变体的培养物中添加外源海藻糖时，该菌株对氧化应激的耐受性增强。真实的时间反转录聚合酶链反应（RT-PCR）分析显示，与cap 1/cap 1突变株相比，野生型菌株中TPS 2和TPS 3的转录水平在暴露于H2 O2时增加。这些结果表明，海藻糖的积累是重要的氧化胁迫耐受介导的Cap 1 p在C。白色念珠菌。
Trehalose, a nonreducing disaccharide which accumulates dramatically during stationary phase or under oxidative stress, is well known as a stress protectant in several organisms. Here we investigated the putative correlation of trehalose with Cap1p, which is a basic region-leucine zipper (bZip) transcription factor participating in oxidative stress tolerance in Candida albicans. HPLC-MS analysis showed that trehalose did not accumulate in the cap1/cap1 mutant during stationary phase. When the mutant was exposed to high concentration of H2O2, trehalose accumulation was still not induced. Under both of the conditions above, the cap1/cap1 mutant showed high sensitivity to H2O2, and the cell viability was rather low. Furthermore, when exogenous trehalose was added to the culture of the cap1/cap1 mutant, the tolerance of this strain to oxidative stress was increased. Real time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcript levels of TPS2 and TPS3 were increased in the wild type strain compared to that in cap1/cap1 mutant when exposed to H2O2. These results indicated that trehalose accumulation is important to the oxidative stress tolerance mediated by Cap1p in C. albicans.