Ultrasensitive electrochemical sensor for prostate specific antigen detection with a phosphorene platform and magnetic covalent organic framework signal amplifier
Ultrasensitive electrochemical sensor for prostate specific antigen detection with a phosphorene platform and magnetic covalent organic framework signal amplifier
复制标题
用于前列腺特异性抗原检测的超灵敏电化学传感器，具有磷烯平台和磁性共价有机骨架信号放大器
DOI：
10.1016/j.bios.2019.111691
复制
发表时间：
2019-11-01
影响因子：
12.6
通讯作者：
Li, Can-Peng
中科院分区：
文献类型：
--
作者：
Liang, Huan;Xu, Hanbin;Li, Can-Peng
Magnetic covalent organic frameworks (COFs) are useful mesoporous materials for the enrichment and separation of analytes, and are utilized in the pretreatment of samples. However, the use of magnetic COFs in electrochemical immunosensors has rarely reported. Herein, a novel electrochemical assay for the determination of prostate specific antigen (PSA) was developed using black phosphorene (BPene) as a platform and magnetic COFs for signal amplification. BPene was prepared via water-phase exfoliation. BPene nanocomposite (Au@BPene) was prepared by depositing Au nanoparticles (Au NPs) onto BPene. This nanocomposite was utilized as an immunsensing platform to bind primary antibodies and improve electron transfer. Subsequently, an Au NP-loaded magnetic COF was used to immobilize the secondary antibodies and abundant electronic signals of methylene blue (MB). The fabricated sensor exhibited linearity ranging from 0.0001 ng mL(-1) to 10 ng with the detection limit of 30 fg mL(-1). The sensor could determine the PSA in a real sample with excellent specificity, good stability, and desirable reproducibility. The effective signal amplification of the proposed sensor is attributed to the good electron transfer of Au@BPene, excellent enrichment capacity of signal molecules (MB) of the COF, and efficient catalytic activity of Fe3O4. This work not only provides an effective electrochemical assay to detect PSA in real sample, but also broadens the utilization scope of magnetic COFs in immunosensing.
