The rice FLATTENED SHOOT MERISTEM, encoding CAF-1 p150 subunit, is required for meristem maintenance by regulating the cell-cycle period
The rice FLATTENED SHOOT MERISTEM, encoding CAF-1 p150 subunit, is required for meristem maintenance by regulating the cell-cycle period
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DOI：
10.1016/j.ydbio.2008.04.040
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发表时间：
2008-07-15
影响因子：
2.7
通讯作者：
Nagato, Yasuo
中科院分区：
文献类型：
--
作者：
Abe, Masashi;Kuroshita, Hideaki;Nagato, Yasuo
我们在水稻中分离出了表现出幼苗生长缺陷并在营养生长期死亡的扁平茎分生组织突变体。由于大多数FSM植物有平坦和小的茎顶端分生组织（SAMs），我们建议FSM是需要适当的SAM维护。FSM编码拟南芥FASCIATA1（FAS 1）的一个假定的直系同源物，其对应于染色质装配因子-1（CAF-1）的p150亚基。FSM在具有活跃分裂细胞的组织中呈斑块状表达，表明FSM与特定细胞周期阶段紧密相关。双靶原位杂交与细胞周期标记基因的复染显示，FSM主要在G，期表达。在fsm中，代表S-和G（2）-至M-期的两个标记基因的表达在SAM中增强，尽管SAM中的细胞数量减少，表明在Jsm中S-和G2-期延长。此外，在JSM叶发育事件发生在适当的时间，表明时间调节的发展发生独立的细胞周期期间。与拟南芥fas1的扁平化表型相反，fsm显示SAM的大小减小。fsm和fas1之间相反的表型表明水稻和拟南芥之间SAM维持的调节不同。(c)2008年爱思唯尔公司All rights reserved.
We isolated flattened shoot meristem (fsm) mutants in rice that showed defective seedling growth and died in the vegetative phase. Since most fsm plants had flat and small shoot apical meristems (SAMs), we suggest that FSM is required for proper SAM maintenance. FSM encodes a putative ortholog of Arabidopsis FASCIATA1 (FAS1) that corresponds to the p150 subunit of chromatin assembly factor-1 (CAF-1). FSM is expressed patchily in tissues with actively dividing cells, suggesting a tight association of FSM with specific cell-cycle phases. Double-target in situ hybridization counterstained with cell-cycle marker genes revealed that FSM is expressed mainly in the G, phase. In fsm, expressions of the two marker genes representing S- and G(2)- to M-phases were enhanced in SAM, despite a reduced number of cells in SAM, suggesting that S- and G2-phases are prolonged in Jsm. In addition, developmental events in Jsm leaves took place at the proper time, indicating that the temporal regulation of development occurs independently of the cell-cycle period. In contrast to the fasciated phenotype of Arabidopsis fas1, fsm showed size reduction of SAM. The opposite phenotypes between fsm and fas1 indicate that the SAM maintenance is regulated differently between rice and Arabidopsis. (c) 2008 Elsevier Inc. All rights reserved.