Lactoferrin: a promoter of polymorphonuclear leukocyte adhesiveness.
Lactoferrin: a promoter of polymorphonuclear leukocyte adhesiveness.
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乳铁蛋白：多形核白细胞粘附性的促进剂。
DOI：
10.1182/blood.v57.5.939.bloodjournal575939
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发表时间：
1981
期刊：
影响因子：
20.3
通讯作者：
L. Boxer
中科院分区：
文献类型：
--
作者：
R. Oseas;H. Yang;R. Baehner;L. Boxer
多形核白细胞(PMN)在暴露于高浓度的趋化刺激(如甲酰-甲硫酰-亮氨酰苯丙氨酸(FMLP))后，脱颗粒、黏附于血管内皮细胞或相互聚集。在趋化刺激下，PMN更容易释放特定的颗粒产物乳铁蛋白，这与通过光传输和增强PMN粘附性来促进PMN聚集有关。刀豆蛋白A(Con-A)和佛波酯(PMA)都能诱导和维持人PMN聚集，它们都能导致特定的颗粒放电。同样，由ConA处理的PMN产生的上清在凝集素的竞争性抑制剂α-甲基甘露糖苷的存在下聚集新鲜的PMN。抗人乳铁蛋白Ig G可抑制PMA和FMLP诱导的聚集，而不是正常山羊Ig G。人乳乳铁蛋白和兔PMN乳铁蛋白聚集人乳铁蛋白均能促进PMN与内皮细胞的黏附。PMN粘附性增强与脱颗粒的早期阶段相关。因此，PMN乳铁蛋白发挥自身调节作用，将PMN保留在炎症部位，以放大炎症反应。
Polymorphonuclear leukocytes (PMN) degranulate, adhere to vascular endothelium, or aggregate to each other following exposure of the cells to high concentrations of chemotactic stimuli such as formyl-methionyl-leucyl phenylalanine (FMLP). PMN released the specific granule product lactoferrin more readily in response to chemotactic stimuli, which correlated with promotion of PMN aggregation as measured by light transmission and enhanced PMN adherence. Both concanavalin A (Con-A) and phorbol myristate acetate (PMA), agents that lead to specific granule discharge, induced and sustained human PMN aggregation. Similarly, supernatants, generated from Con-A-treated PMN, aggregated fresh PMN in the presence of alpha-methylmannoside, a competitive inhibitor of the lectin. Anti-human lactoferrin IgG but not normal goat IgG blunted the aggregation elicited by both PMA and FMLP. Both human milk lactoferrin and rabbit PMN lactoferrin aggregated human lactoferrin promoted PMN adherence to endothelial cells. The enhanced PMN stickiness was correlated with the early phase of degranulation. Thus, PMN lactoferrin serves an autoregulatory role to retain PMN at inflammatory sites to amplify the inflammatory response.