AGING IS ASSOCIATED WITH A DIFFUSE IMPAIRMENT OF FOREBRAIN CHOLINERGIC NEURONS
AGING IS ASSOCIATED WITH A DIFFUSE IMPAIRMENT OF FOREBRAIN CHOLINERGIC NEURONS
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DOI：
10.1016/0006-8993(90)91116-x
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发表时间：
1990-01-29
期刊：
影响因子：
2.9
通讯作者：
ALBANESE, A
中科院分区：
文献类型：
--
作者：
ALTAVISTA, MC;ROSSI, P;ALBANESE, A
该研究评估了自然衰老过程中是否有任何退行性变化影响前脑胆碱能系统。在成年和老年 Wistar 大鼠中研究了内侧间隔核、斜带核、新纹状体和基底核。布彻的乙酰胆碱酯酶技术使我们能够识别位于这些前脑核中的神经元，这些神经元被酶强烈或中等程度地染色，并且被认为具有胆碱能。测量含有染色神经元的前脑区域的大小以及位于其中的染色核周体的数量和大小。在老年大鼠中，前脑胆碱能核的大小平均减少了 26%。老年大鼠中这些区域的神经元密度也显着低于对照组：强烈染色的神经元平均减少 27.81%，而强烈和中度染色的核周细胞总共减少 25.43%。老年大鼠的染色核周横截面积也减少了 32.87%。这些数据表明，老年大鼠前脑中含有乙酰胆碱酯酶的神经元数量减少。它们与自然衰老导致前脑胆碱能核周细胞弥漫性和均匀耗竭的假设是一致的。存活且可选择性染色的神经元表现出形态学改变，这可能与退化过程有关。
The study evaluates whether any degenerative changes affect forebrain cholinergic systems during natural aging. The medial septal nucleus, the nuclei of the diagonal band, the neostriatum, and the basal nucleus were studied in adult and aged Wistar rats. Butcher''s technique for acetylcholinesterase allowed us to identify neurons located in these forebrain nuclei, which stained intensely or moderately for the enzyme and were putatively cholinergic. The size of forebrain regions containing stained neurons, and the number and size of stained perikarya located therein, were measured. In aged rats, the size of forebrain cholinergic nuclei was reduced by an average of 26%. The density of neurons located in these regions was also significantly lower in aged rats than in controls: intensely stained neurons displayed a mean reduction of 27.81%, while intensely and moderately stained perikarya together were reduced by 25.43%. Cross-sectional area of the stained perikarya was also reduced in aged rats by 32.87%. These data show that the number of forebrain acetylcholinesterase-containing neurons is reduced in aged rats. They are consistent with the hypothesis that natural aging brings about a diffuse and homogeneous depletion of forebrain cholinergic perikarya. Neurons which are viable, and can be selectively stained, show morphological alterations, which are likely to be related to a degenerative process.