HLA GENES ASSOCIATED WITH RHEUMATOID-ARTHRITIS - IDENTIFICATION OF SUSCEPTIBILITY ALLELES USING SPECIFIC OLIGONUCLEOTIDE PROBES
HLA GENES ASSOCIATED WITH RHEUMATOID-ARTHRITIS - IDENTIFICATION OF SUSCEPTIBILITY ALLELES USING SPECIFIC OLIGONUCLEOTIDE PROBES
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DOI：
10.1002/anr.1780320104
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发表时间：
1989-01-01
影响因子：
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通讯作者：
NEPOM, BS
中科院分区：
文献类型：
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作者：
NEPOM, GT;BYERS, P;NEPOM, BS
我们使用识别DR 4相关和非DR 4相关基因的寡核苷酸探针，研究了单个HLA基因与类风湿性关节炎（RA）的相关性。两种不同的HLA-DR β。DR 4 + RA患者中Dw 4和Dw 14等位基因的检出率分别为50%和17%，Dw 14等位基因的检出率分别为35%和5%，总DR 4等位基因的检出率分别为73%和30%，表明这两种等位基因是独立的易感基因。值得注意的是，大多数DR 4- RA患者还表现出线性DNA序列，显然是在不同的易感性等位基因之间“洗牌”，用寡核苷酸探针鉴定Dw 14基因的关键部分。
We examined the association of individual HLA genes with rheumatoid arthritis (RA), using oligonucleotide probes that identified both DR4-associated and non-DR4-associated genes. Two distinct HLA-DR.beta. alleles (Dw4 and Dw14) were found in DR4+ RA patients compared with controls (Dw4 50% versus 17%; Dw14 35% versus 5%; total DR4 73% versus 30%), indicating that these 2 alleles are independent susceptibility genes. Remarkably, the majority of the DR4- RA patients also demonstrated a linear DNA sequence, apparently "shuffled" between different susceptibility alleles, identified with an oligonucleotide probe to a key portion of the Dw14 gene.