Characterization of temperature-sensitive strains of Neospora caninum in mice
Characterization of temperature-sensitive strains of Neospora caninum in mice
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DOI：
10.2307/3285701
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发表时间：
1999-02-01
影响因子：
1.3
通讯作者：
Brake, DA
中科院分区：
文献类型：
--
作者：
Lindsay, DS;Lenz, SD;Brake, DA
通过化学诱变和选择在 32°C 下生长，选择了犬新孢子虫速殖子的温度敏感 (ts) 菌株。本研究检查了三种 ts 菌株和亲本犬新孢子虫野生型菌株 NC-1 在近交 BALB/c 小鼠、远交 ICR 小鼠和化学免疫抑制 ICR 小鼠中引起疾病的能力。在 BALB/c 小鼠中，所有 3 种菌株均未能诱发临床疾病，而 NC-1 菌株的感染导致一些小鼠中枢神经系统疾病和死亡。在接种3 ts品系或NC-I品系的ICR小鼠中未观察到疾病。所有接种 NC-1 品系的免疫抑制 ICR 小鼠均死亡，而接种 NCts-4 品系的免疫抑制小鼠均未死亡，接种 NCts-8 和 NCts-12 品系的 5 只小鼠中仅有 1 只死亡。 NCts-4 和 NCts-12 菌株在 37°C 下生长时恢复为野生型表型。用活的而非冷冻的 NCts-8 菌株速殖子对 BALB/c 小鼠进行疫苗接种，在 NC-1 菌株攻击后诱导了显着（P < 0.05）的保护。
Temperature-sensitive (ts) strains of the Neospora caninum tachyzoites were selected by chemical mutagenesis and selection for growth at 32 C. Three ts strains and the parental, N. caninum wild-type strain, NC-1, were examined in the present study for their ability to cause disease in inbred BALB/c mice, outbred ICR mice, and chemically immunosuppressed ICR mice. In BALB/c mice, all 3 strains failed to induce clinical disease, whereas infection with the NC-1 strain caused central nervous system disease and death in some mice. No disease was observed in ICR mice inoculated with the 3 ts strains or the NC-I strain. All immunosuppressed ICR mice inoculated with the NC-1 strain died, whereas no immunosuppressed mice inoculated with the NCts-4 strain and only 1 of 5 mice inoculated with the NCts-8 and NCts-12 strains died. The NCts-4 and NCts-12 strains reverted to a wild-type phenotype when grown at 37 C. Vaccination of BALB/c mice with live, but not frozen NCts-8 strain tachyzoites induced significant (P < 0.05) protection following NC-1 strain challenge.