A cotton germin-like protein GbGLP2 controls fiber length via regulating genes involved in secondary cell wall synthesis
A cotton germin-like protein GbGLP2 controls fiber length via regulating genes involved in secondary cell wall synthesis
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棉花胚芽样蛋白 GbGLP2 通过调节参与次生细胞壁合成的基因来控制纤维长度
DOI：
10.1007/s11032-020-01177-x
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发表时间：
2020-10
影响因子：
3.1
通讯作者：
Lili Tu
中科院分区：
文献类型：
--
作者：
Mengling Sun;Zhengxiu Ye;Jiafu Tan;Sheng Chen;Xianlong Zhang;Lili Tu
棉花是一种天然的纤维作物，也是中国重要的经济作物。培育出纤维更长、更强、更细的棉花一直是棉花育种者的目标。发芽样蛋白（germinlike proteins, GLPs）是一类具有cupin结构域的蛋白家族，主要起酶的作用。然而，glp参与调节棉纤维发育的功能很少被了解。在这里，我们从g中鉴定了一个名为gbglp2的基因。barbadensecDNA文库，并阐明其负调控纤维伸长的功能，导致纤维变短。gbglp2转录本主要富集于花后10天的伸长纤维中。GbGLP2与GhGLP1的相似性为99.8%。同源glp1ing的RNAi沉默。与对照相比，hsutumaccession YZ-1产生的纤维更长。增强gbglp2的表达可显著降低纤维的长度和强度。通过RNAi系与WT系之间的RNA-seq分析，鉴定出566个差异表达基因，其中在RNAi系中下调113个基因，上调453个基因。令人惊讶的是，大多数下调基因参与了次生细胞壁的生物合成，包括纤维素合成酶基因（CESA4、CESA7和cesa8）、半纤维素生物合成基因（IRX9、COBL4）和转录因子（MYB46、MYB86）。同时，对30个纤维较长品种和30个纤维较短品种的转录组比较发现，上述下调基因在长纤维中显著减少。综上所述，这些结果表明，ghglp1的下调通过延迟细胞壁的二次增厚阶段来促进纤维的伸长。
Cotton is not only a natural fiber crop, but also an important economic crop in China. The cultivation of cotton with longer, stronger, and finer fiber has been the goal of cotton breeders. Germin-like proteins (GLPs) is a group of protein family with cupin domain, which mainly acts as an enzyme. However, the function of GLPs involved in regulating cotton fiber development is rarely understood. Here, we identified a gene namedGbGLP2from theG. barbadensecDNA library and elucidated its function negatively regulating fiber elongation that resulted in shorter fiber. The transcripts ofGbGLP2were mainly abundant in the elongating fiber of 10 days post-anthesis (DPA). GbGLP2 shared 99.8% similarity with GhGLP1. RNAi silencing of the homologGhGLP1inG. hirsutumaccession YZ-1 produced longer fiber relative to the control. Enhancing the expression ofGbGLP2significantly reduced fiber length and strength. Through RNA-seq analysis between RNAi and WT lines, 566 genes differentially expressed were identified, of which 113 genes were downregulated and 453 genes were upregulated in the RNAi lines. Surprisingly, most of the downregulated genes participated in secondary cell wall biosynthesis, including cellulose synthase genes (CESA4,CESA7, andCESA8), hemicellulose biosynthesis genes (IRX9,COBL4) and transcription factors (MYB46,MYB86). Simultaneously, the transcriptome comparison between 30 cotton varieties with much longer fiber and 30 varieties with much shorter fiber revealed that the above downregulated genes were markedly decreased in long fiber. Taken together, these results suggest that knockdown ofGhGLP1promotes fiber elongation via delaying secondary cell wall thickening stage.
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DOI：
10.1016/b978-0-12-809633-8.20106-4
发表时间：
2020-02
期刊：
--
影响因子：
--
作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
通讯作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
影响因子：
3.1
作者：
Furong Wang;Zhenzhen Xu;Ran Sun;Yongchao Gong;G. Liu;Jing-xia Zhang;Liuming Wang;Chuanyun Zhang-Chuanyun
通讯作者：
Furong Wang;Zhenzhen Xu;Ran Sun;Yongchao Gong;G. Liu;Jing-xia Zhang;Liuming Wang;Chuanyun Zhang-Chuanyun
DOI：
10.1074/jbc.274.47.33274
发表时间：
1999-11
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
T. Yamahara;Tadahiko Shiono;Takanori Suzuki;Katuyuki Tanaka;S. Takio;Kiichi Sato;S. Yamazaki;T. Satoh
通讯作者：
T. Yamahara;Tadahiko Shiono;Takanori Suzuki;Katuyuki Tanaka;S. Takio;Kiichi Sato;S. Yamazaki;T. Satoh
影响因子：
7.4
作者：
Ruan, YL;Xu, SM;Furbank, RT
通讯作者：
Furbank, RT
影响因子：
7.4
作者：
Wu, Ai-Min;Hornblad, Emma;Marchant, Alan
通讯作者：
Marchant, Alan